Ahmadi Malahat, Adibhesami Masood
Associate Professor of Microbiology, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Iran J Pharm Res. 2017 Spring;16(2):661-669.
The microorganisms have been usually noted as the major cause of delayed wound healing. is the most common pathogen causing these infections. Silver nanoparticles (AgNPs) show ample antibacterial activities. In present study, the effect of AgNPs alone and in combination with tetracycline investigated on inoculated wounds with in mice. Twenty mice anesthetized and full-thickness skin wounds created on back of them and the bacterial suspension added to each wound bed. Wound infection assessed using total count of bacterial load and also wound healing monitored, macroscopically. In all groups treatments applied topically in the wound bed: AgNPs, tetracycline, AgNPs along with tetracycline and normal saline in control group. The tetracycline along with AgNPs achieved 100% wound closure on day 12. In the AgNPs group, the percentage of wound contraction has close figures compared to tetracycline and normal saline as 98, 99 and 79 percent, respectively. By day 12, all of the treated groups with AgNPs, tetracycline and AgNPs along with tetracycline showed decreases in surface bacterial concentration compared with control group. Also, significant decrease (P < 0.001) in deep skin bacterial counts in the AgNPs, tetracycline and AgNPs along with tetracycline compared with control group at any time point. Application of AgNPs along with tetracycline is more effective than AgNPs and tetracycline alone to reduce the bacterial load whilst wound macroscopic contraction increased. These findings support use of the AgNPs in combination with antibacterial medicine for the treatment of infectious skin wounds.
微生物通常被认为是伤口愈合延迟的主要原因。是引起这些感染的最常见病原体。银纳米颗粒（AgNPs）具有充分的抗菌活性。在本研究中，研究了AgNPs单独以及与四环素联合对小鼠接种伤口的影响。将20只小鼠麻醉并在其背部制造全层皮肤伤口，并将细菌悬液添加到每个伤口床中。使用细菌载量总数评估伤口感染情况，并宏观监测伤口愈合情况。在所有组中，将治疗剂局部应用于伤口床：AgNPs、四环素、AgNPs与四环素联合以及对照组使用生理盐水。四环素与AgNPs联合在第12天实现了100%的伤口闭合。在AgNPs组中，伤口收缩百分比与四环素组和生理盐水组相比接近，分别为98%、99%和79%。到第12天，与对照组相比，所有用AgNPs、四环素以及AgNPs与四环素联合治疗的组的表面细菌浓度均降低。此外，在任何时间点，与对照组相比，AgNPs组、四环素组以及AgNPs与四环素联合组的深层皮肤细菌计数均显著降低（P < 0.001）。AgNPs与四环素联合应用在降低细菌载量方面比单独使用AgNPs和四环素更有效，同时伤口宏观收缩增加。这些发现支持将AgNPs与抗菌药物联合用于治疗感染性皮肤伤口。
