Afrin Sadia, Akter Suraiya, Begum Shamima, Hossain Md Nur
Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.
Department of Microbiology, Jagannath University, Dhaka, Bangladesh.
Front Nutr. 2021 Mar 4;8:619506. doi: 10.3389/fnut.2021.619506. eCollection 2021.
This experiment was conducted to characterize potential spp. isolated from mother's milk and infant feces to obtain new and specific probiotic strains. In this study, seven ascendant strains were identified as spp. based on their morphological characteristics and biochemical properties. Among them, only one (C-1) isolate was identified as through BioLog identification. The study further investigated the isolate through probiotic potentiality tests such as pH and bile tolerance, NaCl tolerance test, gastric juice tolerance, antioxidant activity, resistance to hydrogen, reduction of sodium nitrate, antimicrobial activity, and antibiotic susceptibility test. The result showed that the strain is a potential probiotic based on probiotic capability. The identified strain was most acid-tolerant and retained around 80% viability for up to 4 h at pH 1.0 and 2.0. The isolate showed tolerance against up to 1.50% bile concentration and gastric juice and was able to grow 1-6% NaCl concentrations. showed resistance to most antibiotics as well as antagonistic activity against the tested pathogen, good antioxidant properties, reduction of sodium nitrate and HO. The isolate exhibited good intestinal epithelial adhesion properties, and SDS page was performed for secreted protein analysis. Moreover, the strain showed promising cholesterol-lowering properties based on the cholesterol level. This present result indicates that has superior probiotic properties and can be regarded as a potential probiotic candidate.
本实验旨在对从母乳和婴儿粪便中分离出的潜在菌株进行特性分析，以获得新的特异性益生菌菌株。在本研究中，根据七种优势菌株的形态特征和生化特性将其鉴定为特定菌株。其中，只有一株（C-1）分离株通过Biolog鉴定被鉴定为特定菌株。该研究通过益生菌潜力测试，如pH和胆汁耐受性、NaCl耐受性测试、胃液耐受性、抗氧化活性、抗氢性、硝酸钠还原、抗菌活性和抗生素敏感性测试，对该分离株进行了进一步研究。结果表明，基于益生菌能力，该菌株是一种潜在的益生菌。鉴定出的菌株最耐酸，在pH 1.0和2.0条件下长达4小时可保持约80%的活力。该分离株对高达1.50%的胆汁浓度和胃液具有耐受性，并且能够在1-6%的NaCl浓度下生长。该菌株对大多数抗生素具有抗性，对受试病原体具有拮抗活性，具有良好的抗氧化性能、硝酸钠还原能力和抗氢性。该分离株表现出良好的肠道上皮粘附特性，并进行了SDS-PAGE分析以进行分泌蛋白分析。此外，基于胆固醇水平，该菌株显示出有前景的降胆固醇特性。目前的结果表明，该菌株具有优异的益生菌特性，可被视为潜在的益生菌候选菌株。
