MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China.
Medical Technology School, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China.
J Proteome Res. 2021 May 7;20(5):2319-2328. doi: 10.1021/acs.jproteome.0c00847. Epub 2021 Mar 22.
-Cinnamaldehyde (TC) is a widely used food additive, known for its sterilization, disinfection, and antiseptic properties. However, its antibacterial mechanism is not completely understood. In this study, quantitative proteomics was performed to investigate differentially expressed proteins (DEPs) in in response to TC treatment. Bioinformatics analysis suggested aldehyde toxicity, acid stress, oxidative stress, interference of carbohydrate metabolism, energy metabolism, and protein translation as the bactericidal mechanism. BW25113Δ, Δ, Δ, Δ, Δ, Δ, Δg, and Δ were used to investigate the functions of DEPs through biochemical methods. The present study revealed that TC exerts its antibacterial effects by inducing the toxicity of its aldehyde group producing acid stress. These findings will contribute to the application of TC in the antibacterial field.
肉桂醛（TC）是一种广泛使用的食品添加剂，具有杀菌、消毒和防腐作用。然而，其抗菌机制尚不完全清楚。本研究采用定量蛋白质组学技术研究了 TC 处理后对 的差异表达蛋白（DEPs）。生物信息学分析表明，醛毒性、酸应激、氧化应激、干扰碳水化合物代谢、能量代谢和蛋白质翻译可能是其杀菌机制。通过生化方法，利用 BW25113Δ、Δ、Δ、Δ、Δ、Δ、Δg 和 Δ 来研究 DEPs 的功能。本研究表明，TC 通过诱导其醛基产生酸应激的毒性发挥其抗菌作用。这些发现将有助于 TC 在抗菌领域的应用。
