Department of Zoology, Faculty of Science, Stockholm University, Sweden.
Institute of Biotechnology, University of Helsinki, Finland.
Genome Biol Evol. 2021 May 7;13(5). doi: 10.1093/gbe/evab054.
The comma butterfly (Polygonia c-album, Nymphalidae, Lepidoptera) is a model insect species, most notably in the study of phenotypic plasticity and plant-insect coevolutionary interactions. In order to facilitate the integration of genomic tools with a diverse body of ecological and evolutionary research, we assembled the genome of a Swedish comma using 10X sequencing, scaffolding with matepair data, genome polishing, and assignment to linkage groups using a high-density linkage map. The resulting genome is 373 Mb in size, with a scaffold N50 of 11.7 Mb and contig N50 of 11,2Mb. The genome contained 90.1% of single-copy Lepidopteran orthologs in a BUSCO analysis of 5,286 genes. A total of 21,004 gene-models were annotated on the genome using RNA-Seq data from larval and adult tissue in combination with proteins from the Arthropoda database, resulting in a high-quality annotation for which functional annotations were generated. We further documented the quality of the chromosomal assembly via synteny assessment with Melitaea cinxia. The resulting annotated, chromosome-level genome will provide an important resource for investigating coevolutionary dynamics and comparative analyses in Lepidoptera.
逗号蝴蝶（Polygonia c-album，鳞翅目，凤蝶科）是一种模式昆虫物种，尤其在表型可塑性和植物-昆虫协同进化相互作用的研究中。为了促进基因组工具与大量生态和进化研究的整合，我们使用 10X 测序、使用 matepair 数据进行支架构建、基因组精修以及使用高密度连锁图谱将其分配到连锁群，组装了瑞典逗号的基因组。所得基因组大小为 373Mb，支架 N50 为 11.7Mb，连续体 N50 为 11.2Mb。在对 5286 个基因进行的 5,286 个基因的 BUSCO 分析中，该基因组包含 90.1%的单拷贝鳞翅目同源基因。使用来自幼虫和成虫组织的 RNA-Seq 数据以及节肢动物数据库中的蛋白质，总共注释了 21,004 个基因模型，从而生成了具有功能注释的高质量注释。我们还通过与 Melitaea cinxia 的同线性评估进一步记录了染色体组装的质量。所得注释的染色体水平基因组将为研究鳞翅目协同进化动态和比较分析提供重要资源。
