The State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
University of Science and Technology of China, Hefei 230026, People's Republic of China.
Nanotechnology. 2021 May 3;32(30). doi: 10.1088/1361-6528/abf0cb.
Titanium dioxide nanotubes (TNTs) have attracted increasing interest as implantable materials due to their many desirable properties. However, their blood compatibility remains an issue. In this paper, TNTs of different diameters were modified with two types of zwitterionic polymers, poly(sulfobetaine methacrylate) (pSBMA) and poly(carboxybetaine methacrylate) (pCBMA), which were grafted onto the TNTs using ARGET-ATRP (activators regenerated by electron transfer atom transfer radical polymerization) method. Both pSBMA and pCBMA brushes coatings were found to greatly reduce adsorption of bovine serum albumin (BSA) and fibrinogen (Fib) onto the TNTs, showing excellent protein resistance. Moreover, the effects of the surface topography on the amount of protein adsorption were largely suppressed by the polyzwitterion coatings. The conformation of the protein adsorbed to the substrates was analyzed at the molecular level by Fourier-transform infrared reflection spectroscopy (FT-IR), which revealed that the BSA adsorbed on the polyzwitterion-modified TNTs adopted significantly different secondary structures from that on the virgin TNTs, whereas the conformation of the adsorbed Fib remained basically the same. The polyzwitterion-modified TNTs were found to be non-hemolytic, and platelet adhesion and activation was significantly reduced, showing excellent blood compatibility.
二氧化钛纳米管（TNTs）由于其许多理想的特性，作为可植入材料引起了越来越多的关注。然而，它们的血液相容性仍然是一个问题。在本文中，使用 ARGET-ATRP（原子转移自由基聚合引发剂再生电子转移）方法，将两种类型的两性离子聚合物，聚（磺酸甜菜碱甲基丙烯酸酯）（pSBMA）和聚（羧酸甜菜碱甲基丙烯酸酯）（pCBMA）接枝到不同直径的 TNTs 上，对 TNTs 进行了修饰。实验发现，pSBMA 和 pCBMA 刷状涂层都能极大地减少牛血清白蛋白（BSA）和纤维蛋白原（Fib）吸附到 TNTs 上，表现出优异的抗蛋白质能力。此外，聚两性离子涂层在很大程度上抑制了表面形貌对蛋白质吸附量的影响。通过傅里叶变换红外反射光谱（FT-IR）在分子水平上分析了吸附到基底上的蛋白质的构象，结果表明，吸附到聚两性离子修饰的 TNTs 上的 BSA 采用了与原始 TNTs 明显不同的二级结构，而吸附的 Fib 的构象基本保持不变。研究发现，聚两性离子修饰的 TNTs 不具有溶血作用，血小板黏附和激活明显减少，具有优异的血液相容性。
