Ilbeigi Kayhan, Askari Badouei Mahdi, Vaezi Hossein, Zaheri Hassan, Aghasharif Sina, Kafshdouzan Khatereh
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Department of Biomedical Sciences, University of Antwerp, Wilrijk, Belgium.
Department of Pathobiology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran.
BMC Res Notes. 2021 Mar 23;14(1):107. doi: 10.1186/s13104-021-05519-6.
The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran.
A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage of mcr-1 and mcr-2 in Iran until 2016. This study suggests that the other mcr variants (mcr-3 to mcr-9) might be responsible for conferring colistin resistance in animal isolates in Iran. The possible linkage between pig farming industry and high level of mcr carriage in some countries needs to be clarified in future prospective studies.
由于黏菌素是治疗多重耐药革兰氏阴性菌感染的最后一道防线，人畜来源的耐黏菌素肠杆菌科细菌的出现是主要的公共卫生问题之一。我们旨在确定从伊朗的食用动物和伴侣动物中分离出的共生和致病性大肠杆菌菌株中广泛存在的黏菌素耐药原型基因（mcr-1和mcr-2）的流行情况。
总共607株大肠杆菌分离株，这些分离株于2008年至2016年间从不同动物来源收集，用于通过PCR检测质粒介导的黏菌素耐药基因（mcr-1和mcr-2）的可能存在情况。总体而言，我们的结果未能证实在所研究的分离株中存在任何mcr-1或mcr-2阳性大肠杆菌。结论是，尽管食用动物在传播抗生素耐药性方面发挥着重要作用，但直到2016年，它们并非伊朗mcr-1和mcr-2携带的主要来源。这项研究表明，其他mcr变体（mcr-3至mcr-9）可能是伊朗动物分离株中黏菌素耐药性的原因。未来的前瞻性研究需要阐明养猪业与一些国家高水平mcr携带之间可能的联系。
