Yang Jinhui, Liu Xiaoyun, Dai Guangcheng, Qu Lanying, Tan Bo, Zhu Bo, Qi Fuming, Gai Xinyu, Cheng Bo
Urology and Andrology Department, Shengli OilFiled Central Hospital, Dongying, 257034, Shandong, China.
Department of Urology, The Second Affiliated Hospital of Soochow University, 215004, Suzhou, China.
J Cancer. 2021 Mar 1;12(8):2430-2439. doi: 10.7150/jca.53385. eCollection 2021.
Accumulating evidence suggest that circRNA RNAs (circRNAs) play important roles in tumor formation and development. circNT5E has been shown to be an oncogenic gene in several types of cancer, and the high expression of circNT5E lead to tumorigenesis and cancer progression. However, the precise role of circNT5E in bladder cancer (Bca) has not been characterized. In this study, we observed that circNT5E expression was augmented in Bca tissues compared with that in adjacent normal tissues, and its expression level was positively associated with larger tumor size and lower survival rate. Further experiments showed that suppression of circNT5E restrained the growth and metastasis of Bca cells . circNT5E was mainly distributed in the cytoplasm and it captured miR-502-5p to increase HOXC8 mRNA and protein expression. Moreover, decreased miR-502-5p obviously reversed the circNT5E silencing-mediated inhibition of Bca cell growth and migration. Thus, this study suggested that circNT5E may act as a pro-oncogene in the development and progression of Bca and it may become a useful tumor biomarker and promising therapeutic target for Bca treatment.
越来越多的证据表明，环状RNA（circRNAs）在肿瘤的形成和发展中发挥着重要作用。circNT5E已被证明在几种癌症类型中是一种致癌基因，circNT5E的高表达导致肿瘤发生和癌症进展。然而，circNT5E在膀胱癌（Bca）中的精确作用尚未明确。在本研究中，我们观察到与相邻正常组织相比，circNT5E在Bca组织中的表达增加，并且其表达水平与更大的肿瘤大小和更低的生存率呈正相关。进一步的实验表明，抑制circNT5E可抑制Bca细胞的生长和转移。circNT5E主要分布在细胞质中，它捕获miR-502-5p以增加HOXC8 mRNA和蛋白质表达。此外，miR-502-5p的减少明显逆转了circNT5E沉默介导的对Bca细胞生长和迁移的抑制作用。因此，本研究表明circNT5E可能在Bca的发生和发展中作为一种原癌基因起作用，并且它可能成为Bca治疗的有用肿瘤生物标志物和有前景的治疗靶点。
