Imphat Chureeporn, Thongdeeying Pakakrong, Itharat Arunporn, Panthong Sumalee, Makchuchit Sunita, Ooraikul Buncha, Davies Neal M
Graduate School on Applied Thai Traditional Medicine Program, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Evid Based Complement Alternat Med. 2021 Mar 6;2021:5512961. doi: 10.1155/2021/5512961. eCollection 2021.
has been consumed in the diet in northern Thailand and also used as a medicament in ancient scripture for arthropathies. Thus, this study aimed to evaluate the activity of various extracts from differential parts of via inhibition of inflammatory mediators (NO, TNF-, and PGE) in RAW264.7 macrophages. The chemical composition in active extracts was also analyzed by GC/MS. The parts of this plant studied were whole fruits (F), pericarp (P), and seed (O). The methods of extraction included maceration in hexane, 95% ethanol and 50% ethanol, boiling in water, and water distillation. The results demonstrated that the hexane and 95% ethanolic extract from pericarp (PH and P95) and seed essential oil (SO) were the most active extracts. PH and P95 gave the highest inhibition of NO production with IC as 11.99 ± 1.66 g/ml and 15.33 ± 1.05 g/ml, respectively, and they also showed the highest anti-inflammatory effect on TNF- with IC as 36.08 ± 0.55 g/ml and 34.90 ± 2.58 g/ml, respectively. PH and P95 also showed the highest inhibitory effect on PGE but less than SO with IC as 13.72 ± 0.81 g/ml, 12.26 ± 0.71 g/ml, and 8.61 ± 2.23 g/ml, respectively. 2,3-Pinanediol was the major anti-inflammatory compound analyzed in PH (11.28%) and P95 (19.82%) while terpinen-4-ol constituted a major anti-inflammatory compound in SO at 35.13%. These findings are the first supportive data for ethnomedical use for analgesic and anti-inflammatory activity in acute (SO) and chronic (PH and P95) inflammation.
在泰国北部的饮食中已被食用，并且在古代经文中也被用作治疗关节病的药物。因此，本研究旨在通过抑制RAW264.7巨噬细胞中的炎症介质（NO、TNF-α和PGE）来评估该植物不同部位的各种提取物的活性。还通过GC/MS分析了活性提取物中的化学成分。研究的该植物部位有整果（F）、果皮（P）和种子（O）。提取方法包括在己烷、95%乙醇和50%乙醇中浸渍、水煮和水蒸馏。结果表明，果皮的己烷提取物和95%乙醇提取物（PH和P95）以及种子精油（SO）是活性最高的提取物。PH和P95对NO生成的抑制作用最强，IC50分别为11.99±1.66μg/ml和15.33±1.05μg/ml，它们对TNF-α也显示出最高的抗炎作用，IC50分别为36.08±0.55μg/ml和34.90±2.58μg/ml。PH和P95对PGE的抑制作用也最强，但低于SO，IC50分别为13.72±0.81μg/ml、12.26±0.71μg/ml和8.61±2.23μg/ml。2,3-蒎烷二醇是在PH（11.28%）和P95（19.82%）中分析出的主要抗炎化合物，而萜品-4-醇是SO中的主要抗炎化合物，含量为35.13%。这些发现是该植物在急性（SO）和慢性（PH和P95）炎症中用于止痛和抗炎活性的民族医学用途的首批支持性数据。
