State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Kunming, Yunnan 650224, China.
Yunnan Key Laboratory of Plateau Fish Breeding, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650224, China.
Zool Res. 2021 May 18;42(3):262-266. doi: 10.24272/j.issn.2095-8137.2020.321.
The Dianchi golden-line barbel, (Regan, 1904), is one of the "Four Famous Fishes" of Yunnan Province, China. Given its economic value, this species has been artificially bred successfully since 2007, with a nationally selected breed (" , Bayou No. 1") certified in 2018. For the future utilization of this species, its growth rate, disease resistance, and wild adaptability need to be improved, which could be achieved with the help of molecular marker-assisted selection (MAS). In the current study, we constructed the first chromosome-level genome of , assembled 48 pseudo-chromosomes, and obtained a genome assembly of 1.49 Gb. We also performed QTL-seq analysis of using the highest and lowest bulks (i.e., largest and smallest size) in both a sibling and random population. We screened two quantitative trait loci (QTLs) (Chr3, 14.9-39.1 Mb and Chr17, 4.1-27.4 Mb) as the major growth-related locations. Several candidate genes (e.g., , , , , and ) were also identified, with functions related to growth, such as cell differentiation, neuronal development, skeletal muscle development, chondrogenesis, and immunity. These results built a solid foundation for in-depth MAS studies on the growth traits of .
滇池金线鲃，（Regan，1904），是中国云南省“四大名鱼”之一。由于其经济价值，自 2007 年以来，该物种已成功进行人工养殖，2018 年获得国家选育品种（“Bayou No.1”）认证。为了未来利用该物种，需要提高其生长速度、抗病能力和野生适应性，这可以通过分子标记辅助选择（MAS）来实现。在本研究中，我们构建了滇池金线鲃的第一个染色体水平基因组，组装了 48 条假染色体，获得了 1.49 Gb 的基因组组装。我们还使用同胞和随机群体中最大和最小大小的最高和最低两个群体进行了 QTL-seq 分析。我们筛选了两个数量性状位点（QTL）（Chr3，14.9-39.1 Mb 和 Chr17，4.1-27.4 Mb）作为主要的生长相关位置。还鉴定了几个候选基因（例如、、、、和），它们的功能与生长有关，如细胞分化、神经元发育、骨骼肌发育、软骨形成和免疫。这些结果为深入研究滇池金线鲃的生长性状奠定了坚实的 MAS 基础。
