Yin Yanhui, Zhang Yuanwei, Hua Zexiang, Wu Anli, Pan Xiaofu, Yang Junxing, Wang Xiaoai
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Kunming, Yunnan, China.
Yunnan Key Laboratory of Plateau Fish Breeding, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
Front Genet. 2023 Jul 19;14:1217952. doi: 10.3389/fgene.2023.1217952. eCollection 2023.
is an economically valuable and famous fish in Yunnan Province, China. However, given its slow growth (40 g/2 years) and large growth differences among individuals, its growth performance needs to be improved for sustainable future use, in which molecular breeding technology can play an important role. In the current study, we conducted muscle transcriptomic analysis to investigate the growth gaps among individuals and the mechanism underlying growth within 14 fast- and 14 slow-growth . In total, 1,647 differentially expressed genes (DEGs) were obtained, including 947 up-regulated and 700 down-regulated DEGs in fast-growth group. Most DEGs were significantly enriched in ECM-receptor interaction, starch and sucrose metabolism, glycolysis/gluconeogenesis, pyruvate metabolism, amino acids biosynthesis and metabolism, peroxisome, and PPAR signaling pathway. Some genes related to glycogen degradation, glucose transport, and glycolysis (e.g., , , , , , , , , , , , , and ) were up-regulated, while some genes related to fatty acid degradation and transport (e.g., , , , , and ) and amino acid metabolism (e.g., , , , and ) were down-regulated in the fast-growth group. Weighted gene co-expression network analysis identified , , , , , , , and as crucial genes for growth. Several genes related to bone and muscle growth (e.g., , , , , and ) were also up-regulated in the fast-growth group. These results suggest that fast-growth fish may uptake adequate energy (e.g., glucose, fatty acid, and amino acids) from fodder, with excess energy substances used to synthesize collagen to accelerate bone and muscle growth after normal life activities are maintained. Moreover, energy uptake may be the root cause, while collagen synthesis may be the direct reason for the growth gap between fast- and slow-growth fish. Hence, improving food intake and collagen synthesis may be crucial for accelerating growth, and further research is required to fully understand and confirm these associations.
是中国云南省一种具有经济价值的著名鱼类。然而，鉴于其生长缓慢（40克/2年）且个体间生长差异较大，为了未来可持续利用，其生长性能需要提高，分子育种技术在其中可发挥重要作用。在当前研究中，我们进行了肌肉转录组分析，以研究14条快速生长和14条慢速生长个体之间的生长差距以及生长的潜在机制。总共获得了1647个差异表达基因（DEGs），其中快速生长组中有947个上调DEGs和700个下调DEGs。大多数DEGs在细胞外基质-受体相互作用、淀粉和蔗糖代谢、糖酵解/糖异生、丙酮酸代谢、氨基酸生物合成和代谢、过氧化物酶体以及PPAR信号通路中显著富集。一些与糖原降解、葡萄糖转运和糖酵解相关的基因（如……）在快速生长组中上调，而一些与脂肪酸降解和转运（如……）以及氨基酸代谢（如……）相关的基因在快速生长组中下调。加权基因共表达网络分析确定……为生长的关键基因。一些与骨骼和肌肉生长相关的基因（如……）在快速生长组中也上调。这些结果表明，快速生长的鱼可能从饲料中摄取足够的能量（如葡萄糖、脂肪酸和氨基酸），在维持正常生命活动后，多余的能量物质用于合成胶原蛋白以加速骨骼和肌肉生长。此外，能量摄取可能是根本原因，而胶原蛋白合成可能是快速生长和慢速生长鱼之间生长差距的直接原因。因此，提高食物摄入量和胶原蛋白合成可能对加速……生长至关重要，需要进一步研究以充分理解和证实这些关联。
