Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, 43600, Bangi, Selangor, Malaysia.
Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM, 43600, Bangi, Selangor, Malaysia.
BMC Res Notes. 2021 Mar 25;14(1):117. doi: 10.1186/s13104-021-05532-9.
Agarwood is the aromatic heartwood formed upon wounding of Aquilaria trees either naturally formed due to physical wound sustained from natural phenomena followed by microbial infection, or artificially induced using different inoculation methods. Different induction methods produce agarwoods with different aromas which have impacts on their commercial values. In lieu of elucidating the molecular mechanisms of agarwood formation under different treatment conditions, the transcriptome profiles of trunk tissues from healthy A. malaccensis tree, and naturally and artificially induced trees were obtained.
The transcriptome of trunk tissues from healthy A. malaccensis, and naturally and artificially induced trees were sequenced using Illumina HiSeq™ 4000 platform which resulted in a total of 38.4 Gb clean reads with Q30 rate of at least 91%. The transcriptome consists of 85,986 unigenes containing 1305 bases on average which were annotated against several databases. From this, 44,654 unigenes were mapped to 290 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes database. These transcriptome data represent considerable contribution towards Aquilaria transcriptome data and enhance current knowledge in comprehending the molecular mechanisms underlying agarwood formation in Aquilaria spp.
沉香木是由沉香树在自然条件下(由于自然现象导致的物理损伤,随后受到微生物感染)或通过不同的接种方法(人工诱导)形成的芳香心材。不同的诱导方法会产生具有不同香气的沉香木,从而影响其商业价值。为了阐明不同处理条件下沉香木形成的分子机制,我们获得了健康的白木香树以及自然和人工诱导的树木的树干组织的转录组图谱。
使用 Illumina HiSeq™ 4000 平台对健康的白木香树、自然和人工诱导的树木的树干组织进行转录组测序,共获得 38.4GB 的清洁读数,Q30 率至少为 91%。转录组由 85986 条平均包含 1305 个碱基的 unigene 组成,这些 unigene 被注释到几个数据库中。其中,44654 条 unigene 被映射到京都基因与基因组百科全书数据库中的 290 条代谢途径。这些转录组数据为沉香属的转录组数据做出了重要贡献,并增强了我们对沉香属树木中沉香木形成的分子机制的理解。