Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Haikou, Hainan, China.
PLoS One. 2018 Jun 1;13(6):e0198111. doi: 10.1371/journal.pone.0198111. eCollection 2018.
Only when Aquilaria spp. or Gyrinops spp. trees are wounded, due to insect attack, or microbial invasion, agarwood can be successfully induced. In the present study, a fungus which can induce agarwood formation efficiently was isolated and a suitable method for its application to induce agarwood formation was developed. Rigidoporus vinctus was isolated from the inner layers from infectious A. sinensis trees. When the fermentation liquid of fungi inoculated back to A. sinensis tree, agarwood was found to be induced. In addition, a novel method called trunk surface agarwood-inducing technique (Agar-Sit) was developed to produce agarwood with R. vinctus. The alcohol soluble extract content of the agarwood, up to 38.9%, far higher than the requirement (10%) in Chinese Pharmacopoeia and the six characteristic compounds of agarwood used as Chinese Medicinal Materials were all detected. Their relative percentages of the sesquiterpenes in the essential oil were 22.76%. This is the first report of the Agar-Sit and also the application of R. vinctus in agarwood induction. According to the results, when the combination of Agar-Sit and R. vinctus is used agarwood can be induced with high yield and good quality.
只有当沉香属（Aquilaria spp.）或黄杞属（Gyrinops spp.）树木受伤时，例如受到昆虫攻击或微生物入侵，才能成功诱导沉香的形成。在本研究中，我们分离出一种能够高效诱导沉香形成的真菌，并开发出一种合适的方法将其应用于诱导沉香形成。从受感染的沉香树的内层中分离出硬孔菌（Rigidoporus vinctus）。当将真菌的发酵液接种回沉香树时，发现沉香树能够诱导产生沉香。此外，我们还开发了一种名为树干表面沉香诱导技术（Agar-Sit）的新方法，利用硬孔菌（Rigidoporus vinctus）来生产沉香。所产生的沉香的醇溶性浸出物含量高达 38.9%，远高于中国药典（Chinese Pharmacopoeia）规定的 10%的要求，并且检测到了作为中药材使用的沉香的 6 种特征化合物。它们在精油中的倍半萜相对百分比为 22.76%。这是首次报道 Agar-Sit 技术，也是硬孔菌（Rigidoporus vinctus）在沉香诱导方面的首次应用。根据研究结果，当采用 Agar-Sit 与硬孔菌（Rigidoporus vinctus）相结合的方法时，可以实现高产、高质量的沉香诱导。
