Deng Kejun, Lan Xiuhua, Fang Qing, Li Mengke, Xie Guangbo, Xie Liyuan
School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
Front Mol Biosci. 2021 Mar 9;8:632341. doi: 10.3389/fmolb.2021.632341. eCollection 2021.
one of the true morels, has recently been artificially cultivated with stable production in China. Analysis of the variations in primary metabolites during the vegetative stages of is critical for understanding the metabolic process. In this study, three developmental stages were categorized based on morphological and developmental changes, including the young mushroom period, marketable mature period, and physiological maturity period. Untargeted metabolomics-based mass spectrometry was used to analyze the change of metabolites during the growth stages of . The result showed that the metabolites' content at the different growth stages were significantly different. The relative contents of linoleic acid, mannitol, oleamide, and betaine were higher at each growth stage. Flavor substances were significantly metabolizable during commodity maturity, while amino acids, organic acids, and lipids were significantly metabolizing at physiological maturity. Pathway analysis of the most significant changes involved Pyrimidine metabolism, Vitamin B6 metabolism, Arginine biosynthesis, Lysine biosynthesis, and Lysine degradation. The results can provide a theoretical basis for further clarifying the metabolic regulation mechanism and lay the foundation for optimizing the cultivation process of .
一种真正的羊肚菌，最近在中国已实现人工栽培且产量稳定。分析羊肚菌营养生长阶段初级代谢产物的变化对于理解其代谢过程至关重要。在本研究中，根据形态和发育变化将其分为三个发育阶段，包括幼菇期、可上市成熟期和生理成熟期。采用基于非靶向代谢组学的质谱法分析羊肚菌生长阶段代谢产物的变化。结果表明，不同生长阶段代谢产物的含量存在显著差异。亚油酸、甘露醇、油酰胺和甜菜碱在每个生长阶段的相对含量较高。风味物质在商品成熟期间显著可代谢，而氨基酸、有机酸和脂质在生理成熟时显著代谢。对最显著变化的通路分析涉及嘧啶代谢、维生素B6代谢、精氨酸生物合成、赖氨酸生物合成和赖氨酸降解。这些结果可为进一步阐明代谢调控机制提供理论依据，并为优化羊肚菌栽培过程奠定基础。
