Karmakar Sumon, Billah Mutasim, Hasan Mahedi, Sohan Sohanur Rahman, Hossain Md Forhad, Faisal Hoque Kazi Md, Kabir Ahmad Humayan, Rashid Md Mamunur, Talukder Mamunur Rashid, Reza Md Abu
Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.
Molecular Plant Physiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
Heliyon. 2021 Mar 16;7(3):e06458. doi: 10.1016/j.heliyon.2021.e06458. eCollection 2021 Mar.
In this present study conducted with the LFGD (Low-Frequency Glow Discharge) (Ar + O) plasma treated maize seeds, to inspect the effect on seed surface modifications, seed germination, growth, development, productivity and nutritional compositions of maize plants. This study reported that LFGD (Ar + O) plasma treated maize seeds have a potential effect to change its smooth seed surfaces and, it becomes rougher. It also enhances the seed germination rate up to (15.88%), which might help to increase the shoot length (33.42%), root length (10.67%), stem diameter (13.37%), total chlorophyll content (46.93%), total soluble protein (52.48%), total soluble phenol (21.68%) and sugar (1.62%) concentrations in respect controls of our experimental plants. For this reason, the acceptable treatment duration for maize seeds were 30sec, 60sec, 90sec and 120sec. After treatment, the plants exhibited a significant increase in CAT, SOD, APX and GR activities in the leaves and roots, and also significantly changes in HO (208.33 ± 5.87μ molg FW) in the leaves and (61.13 ± 1.72μ molg FW) in the roots, NO was (369.24 ± 213.19μ molgFW) and (1094.23 ± 135.44μ molgFW) in the leaves and roots. LFGD plasma treatment also contributed to enhancement of productivity (1.27%), nutritional (moisture, ash, fat, and crude fiber) compositions, and iron and zinc micro-nutrition concentrations of maize. From this research, LFGD (Ar + O) plasma treatment showed a potential impact on the maize cultivation system, which is very effective tools and both in nationally and internationally alter the conventional cultivation system of maize. Because it promotes seed surface modification, improved germination rate, shoot length, root length, chlorophyll content, some of the growths related enzymatic activity, nutrient composition, iron, and zinc micro-nutrients and the productivity of maize.
在本研究中，对经低频辉光放电（LFGD）（氩气+氧气）等离子体处理的玉米种子进行了研究，以考察其对种子表面改性、种子萌发、生长、发育、生产力及玉米植株营养成分的影响。本研究报告称，LFGD（氩气+氧气）等离子体处理的玉米种子有改变其光滑种子表面的潜在作用，使其变得更粗糙。它还能将种子发芽率提高至（15.88%），这可能有助于增加我们实验植株对照的地上部分长度（33.42%）、根长（10.67%）、茎直径（13.37%）、总叶绿素含量（46.93%）、总可溶性蛋白（52.48%）、总可溶性酚（21.68%）和糖（1.62%）浓度。因此，玉米种子的可接受处理时间为30秒、60秒、90秒和120秒。处理后，植株叶片和根部的CAT、SOD、APX和GR活性显著增加，叶片中HO（208.33±5.87μmolg FW）和根部中HO（61.13±1.72μmolg FW）也有显著变化，叶片中NO为（369.24±213.19μmolgFW），根部中NO为（1094.23±135.44μmolgFW）。LFGD等离子体处理还有助于提高玉米的生产力（1.27%）、营养成分（水分、灰分、脂肪和粗纤维）以及铁和锌的微量营养浓度。从这项研究来看，LFGD（氩气+氧气）等离子体处理对玉米种植系统显示出潜在影响，这是一种非常有效的工具，在国内和国际上都能改变玉米的传统种植系统。因为它促进种子表面改性、提高发芽率、地上部分长度、根长、叶绿素含量、一些与生长相关的酶活性、营养成分、铁和锌微量营养素以及玉米的生产力。
