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姜黄素通过重新表达 JAK/STAT 负调节剂抑制 HL60 白血病细胞的增殖并增强其凋亡。

Thymoquinone Suppresses Cell Proliferation and Enhances Apoptosis of HL60 Leukemia Cells through Re-Expression of JAK/STAT Negative Regulators.

机构信息

School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin (UniSZA), 20300 Terengganu, Malaysia.

Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.

出版信息

Asian Pac J Cancer Prev. 2021 Mar 1;22(3):879-885. doi: 10.31557/APJCP.2021.22.3.879.

DOI:10.31557/APJCP.2021.22.3.879
PMID:33773553
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8286695/
Abstract

OBJECTIVE

The natural compound, thymoquinone (TQ) has demonstrated potential anticancer properties in inhibiting cell proliferation and promoting apoptosis in myeloid leukemia cells, breast cancer cells, and others. However, the effect mechanism of TQ on AML cells still not fully understood. In this study, the authors examined the effects of TQ on the expression of JAK/STAT-negative regulator genes SOCS-1, SOCS-3, and SHP-1, and their consequences on cell proliferation and apoptosis in HL60 leukemia cells.

METHODS

MTT and trypan blue exclusion tests were conducted to determine the 50% inhibitory concentration (IC50) and cell proliferation. FITC Annexin and Guava® reagent were used to study the cell apoptosis and examine the cell cycle phases, respectively. The expression of JAK/STAT-negative regulator genes, SOCS-1, SOCS-3, and SHP-1, was investigated using reverse transcriptase- quantitative PCR (RT-qPCR).

RESULTS

TQ demonstrated a potential inhibition of HL60 cell proliferation and a significant increase in apoptotic cells in dose and time-dependent manner. TQ significantly induced cycle arrest at G0-G1 phase (P < 0.001) and enhanced the re-expression of JAK/STAT-negative regulator genes.

CONCLUSION

TQ potentially inhibited HL60 cell proliferation and significantly increased apoptosis with re-expression of JAK/STAT-negative regulator genes suggesting that TQ could be a new therapeutic candidate for leukemia therapy.
.

摘要

目的

天然化合物，百里醌（TQ）已被证明具有抑制髓系白血病细胞、乳腺癌细胞等细胞增殖和促进细胞凋亡的潜在抗癌特性。然而，TQ 对 AML 细胞的作用机制仍不完全清楚。在这项研究中，作者研究了 TQ 对 JAK/STAT 负调节剂基因 SOCS-1、SOCS-3 和 SHP-1 表达的影响，以及它们对 HL60 白血病细胞增殖和凋亡的影响。

方法

采用 MTT 和台盼蓝排除试验测定 50%抑制浓度（IC50）和细胞增殖。FITC Annexin 和 Guava®试剂分别用于研究细胞凋亡和检查细胞周期阶段。采用逆转录定量 PCR（RT-qPCR）研究 JAK/STAT 负调节剂基因 SOCS-1、SOCS-3 和 SHP-1 的表达。

结果

TQ 表现出抑制 HL60 细胞增殖的潜力，并以剂量和时间依赖的方式显著增加凋亡细胞。TQ 显著诱导 G0-G1 期细胞周期停滞（P<0.001），并增强 JAK/STAT 负调节剂基因的重新表达。

结论

TQ 可能抑制 HL60 细胞增殖，显著增加凋亡，并重新表达 JAK/STAT 负调节剂基因，表明 TQ 可能成为白血病治疗的新治疗候选药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/9a9335ca9882/APJCP-22-879-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/e5218a17fbe8/APJCP-22-879-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/0cedf6fc2e2a/APJCP-22-879-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/a860f1765007/APJCP-22-879-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/9a9335ca9882/APJCP-22-879-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/e5218a17fbe8/APJCP-22-879-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/0cedf6fc2e2a/APJCP-22-879-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/a860f1765007/APJCP-22-879-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7ed/8286695/9a9335ca9882/APJCP-22-879-g004.jpg

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本文引用的文献

1
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Adv Exp Med Biol. 2019;1152:271-281. doi: 10.1007/978-3-030-20301-6_14.
2
Thymoquinone-induced antitumor and apoptosis in human lung adenocarcinoma cells.胸腺醌诱导人肺腺癌细胞的抗肿瘤和凋亡。
J Cell Physiol. 2019 Jul;234(7):10421-10431. doi: 10.1002/jcp.27710. Epub 2018 Nov 1.
3
Re-Expression of Bone Marrow Proteoglycan-2 by 5-Azacytidine is associated with STAT3 Inactivation and Sensitivity Response to Imatinib in Resistant CML Cells.
解析长链非编码RNA在急性髓系白血病治疗耐药中的作用：新前景与挑战
Noncoding RNA Res. 2024 May 20;9(4):1203-1221. doi: 10.1016/j.ncrna.2024.05.009. eCollection 2024 Dec.
4
Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer.百里醌：一种用于治疗结直肠癌的有前景的治疗药物。
Curr Issues Mol Biol. 2023 Dec 23;46(1):121-139. doi: 10.3390/cimb46010010.
5
. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells.内核乙醇提取物通过诱导肺癌细胞的细胞周期停滞和凋亡来抑制细胞活力和增殖。
Mol Cell Oncol. 2024 Jan 4;11(1):2299046. doi: 10.1080/23723556.2023.2299046. eCollection 2024.
6
Potential anticancer properties and mechanisms of thymoquinone in colorectal cancer.黑种草醌在结直肠癌中的潜在抗癌特性及机制
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10
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Pharmaceutics. 2023 Apr 4;15(4):1143. doi: 10.3390/pharmaceutics15041143.
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Asian Pac J Cancer Prev. 2018 Jun 25;19(6):1585-1590. doi: 10.22034/APJCP.2018.19.6.1585.
4
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5
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6
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Bone marrow niche-mediated survival of leukemia stem cells in acute myeloid leukemia: Yin and Yang.急性髓系白血病中骨髓微环境介导的白血病干细胞存活：阴阳学说
Cancer Biol Med. 2016 Jun;13(2):248-59. doi: 10.20892/j.issn.2095-3941.2016.0023.