Abo-Aziza Faten A M, Zaki Abdel Kader A, Alajaji Ahmed I, Al Barrak Saleh M
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Cairo, Egypt.
Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Vet World. 2021 Feb;14(2):347-363. doi: 10.14202/vetworld.2021.347-363. Epub 2021 Feb 8.
is a bovine intestinal nematode. Immune pictures following infection are conflicting and stopping anthelmintic albendazole treatment recording reversed liver abnormalities. The purpose of this work was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells (BMMSCs) therapy, subsequent to albendazole administration in rats infected with .
The ultrasonographic and histopathological examinations as well as serum liver enzymes activity and the kinetics of recovery were investigated. The correlation of cell-mediated and humoral immune pictures was assessed by assaying immunoglobulins, splenocytes viability, phagocytic index, and Th1/Th2 cytokines.
The cultured BMMSCs counting were 4.21×10 cells/cm with 96.03% viability. Flow-cytometric analysis indicated positive CD90 (82%), CD105 (79%) and negative CD34 (0.37%), CD45 (0.42%), attesting to the suitability of the isolated BMMSCs for use in therapy. Transplantation of BMMSCs after albendazole administration significantly reduced the release of liver enzymes (p<0.05) indicating liver cellularity improvement. The ultrasonographic, macroscopic, and histopathological findings confirmed the biochemical results. Significant elevation in the levels of tumor necrosis factor (TNF)-α and interferon (INF)-γ with a decline in interleukin (IL)-4 was observed in the untreated model (p<0.05). However, albendazole treatment followed by BMMSCs therapy significantly lowered the release of TNF-α and INF-γ, associated with significant production of IL-4 and IL-10 (p<0.05).
The final results indicated that the liver functions, histopathological findings, and immune parameters were aggravated after experimental infection. Albendazole treatment followed by BMMSCs therapy was found to assist in regeneration of injured hepatic tissue. Besides, it appeared to modulate host defensive immune responses against antigens. This work could define more clearly the events that manipulate the host immune, histopathological, and biochemical responses to minimize obstacles in using stem cell therapy in animal toxocariosis.
[寄生虫名称]是一种牛肠道线虫。感染后的免疫情况存在矛盾，停止使用驱虫药阿苯达唑治疗后肝脏异常情况有所逆转。本研究的目的是评估骨髓间充质干细胞（BMMSCs）疗法在感染[寄生虫名称]的大鼠中给予阿苯达唑后的治疗潜力。
研究了超声检查、组织病理学检查以及血清肝酶活性和恢复动力学。通过检测免疫球蛋白、脾细胞活力、吞噬指数和Th1/Th2细胞因子来评估细胞介导免疫和体液免疫情况的相关性。
培养的BMMSCs计数为4.21×10个细胞/cm，活力为96.03%。流式细胞术分析表明CD90呈阳性（82%），CD105呈阳性（79%），而CD34呈阴性（0.37%），CD45呈阴性（0.42%），证明分离出的BMMSCs适合用于治疗。阿苯达唑给药后移植BMMSCs显著降低了肝酶的释放（p<0.05），表明肝细胞情况有所改善。超声、大体和组织病理学检查结果证实了生化结果。在未治疗的模型中观察到肿瘤坏死因子（TNF）-α和干扰素（INF）-γ水平显著升高，而白细胞介素（IL）-4水平下降（p<0.05）。然而，阿苯达唑治疗后再进行BMMSCs疗法显著降低了TNF-α和INF-γ的释放，同时IL-4和IL-10大量产生（p<0.05）。
最终结果表明，实验性感染[寄生虫名称]后肝功能、组织病理学检查结果和免疫参数均恶化。阿苯达唑治疗后再进行BMMSCs疗法有助于受损肝组织的再生。此外，它似乎能调节宿主针对[寄生虫名称]抗原的防御性免疫反应。这项工作可以更清楚地界定在动物弓蛔虫病中利用干细胞疗法时操控宿主免疫、组织病理学和生化反应以尽量减少障碍的相关事件。
