Peng Yuan, He Danfeng, Ge Xin, Lu Yifei, Chai Yuanhao, Zhang Yixin, Mao Zhengwei, Luo Gaoxing, Deng Jun, Zhang Yan
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai, 200011, China.
Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Bioact Mater. 2021 Mar 9;6(10):3109-3124. doi: 10.1016/j.bioactmat.2021.02.006. eCollection 2021 Oct.
Excessive production of inflammatory chemokines and reactive oxygen species (ROS) can cause a feedback cycle of inflammation response that has a negative effect on cutaneous wound healing. The use of wound-dressing materials that simultaneously absorb chemokines and scavenge ROS constitutes a novel 'weeding and uprooting' treatment strategy for inflammatory conditions. In the present study, a composite hydrogel comprising an amine-functionalized star-shaped polyethylene glycol (starPEG) and heparin for chemokine sequestration as well as CuO ultrasmall nanozymes for ROS scavenging (CuO@Hep-PEG) was developed. The material effectively adsorbs the inflammatory chemokines monocyte chemoattractant protein-1 and interleukin-8, decreasing the migratory activity of macrophages and neutrophils. Furthermore, it scavenges the ROS in wound fluids to mitigate oxidative stress, and the sustained release of CuO promotes angiogenesis. In acute wounds and impaired-healing wounds (diabetic wounds), CuO@Hep-PEG hydrogels outperform the standard-of-care product Promogram® in terms of inflammation reduction, increased epidermis regeneration, vascularization, and wound closure.
炎症趋化因子和活性氧（ROS）的过度产生可导致炎症反应的反馈循环，对皮肤伤口愈合产生负面影响。使用同时吸收趋化因子和清除ROS的伤口敷料材料构成了一种针对炎症状况的新型“除草除根”治疗策略。在本研究中，开发了一种复合水凝胶，其包含用于趋化因子螯合的胺官能化星形聚乙二醇（starPEG）和肝素以及用于ROS清除的CuO超小纳米酶（CuO@Hep-PEG）。该材料有效吸附炎症趋化因子单核细胞趋化蛋白-1和白细胞介素-8，降低巨噬细胞和中性粒细胞的迁移活性。此外，它清除伤口液中的ROS以减轻氧化应激，并且CuO的持续释放促进血管生成。在急性伤口和愈合受损的伤口（糖尿病伤口）中，CuO@Hep-PEG水凝胶在减轻炎症、增加表皮再生、血管化和伤口闭合方面优于护理标准产品Promogram®。
