Hou Biao, Li Chengyuan, Yang Fen, Deng Wanjun, Hu Chao, Liu Changxiong, Chen Yanming, Xiao Xiangjun, Huang Xiongjie, Deng Jun, Xie Songlin
Department of Hand and Foot Microsurgery, The Affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang, Hunan, People's Republic of China.
Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, People's Republic of China.
Int J Nanomedicine. 2024 Dec 19;19:13563-13578. doi: 10.2147/IJN.S487647. eCollection 2024.
Stem cell therapy is a promising approach for treating chronic diabetic wounds. However, its effectiveness is significantly limited by the high oxidative stress environment and persistent inflammation induced by diabetes. Strategies to overcome these challenges are essential to enhance the therapeutic potential of stem cell therapy.
CuO ultrasmall nanoparticles (CuO-USNPs), known for their excellent reactive oxygen species (ROS) scavenging properties, were utilized to protect adipose-derived stem cells (ADSCs) from oxidative stress injury. In vitro experiments were conducted to evaluate the viability, paracrine activity, and anti-inflammatory capabilities of ADSCs loaded with CuO-USNPs under oxidative stress conditions. In vivo experiments in diabetic mice were performed to assess the therapeutic effects of CuO-USNP-loaded ADSCs on wound healing, including their impact on inflammation, collagen synthesis, angiogenesis, and wound closure.
ADSCs treated with CuO-USNPs showed significantly enhanced viability, paracrine activity, and anti-inflammatory properties under oxidative stress conditions in vitro. In diabetic mice, CuO-USNP-loaded ADSCs reduced inflammatory responses in wound tissues, promoted collagen synthesis and angiogenesis, and accelerated diabetic wound healing. These findings suggest that CuO-USNPs effectively mitigate the adverse effects of oxidative stress and inflammation, enhancing the therapeutic efficacy of ADSCs.
This study presents a simple and effective approach to improve the therapeutic potential of stem cell therapy for diabetic wounds. By incorporating CuO-USNPs, the antioxidative and anti-inflammatory capabilities of ADSCs are significantly enhanced, offering a promising strategy for ROS-related tissue repair and chronic wound healing.
干细胞疗法是治疗慢性糖尿病伤口的一种有前景的方法。然而，其有效性受到糖尿病所诱导的高氧化应激环境和持续性炎症的显著限制。克服这些挑战的策略对于提高干细胞疗法的治疗潜力至关重要。
氧化铜超小纳米颗粒（CuO-USNPs）以其优异的活性氧（ROS）清除特性而闻名，被用于保护脂肪来源干细胞（ADSCs）免受氧化应激损伤。进行体外实验以评估在氧化应激条件下负载CuO-USNPs的ADSCs的活力、旁分泌活性和抗炎能力。在糖尿病小鼠中进行体内实验，以评估负载CuO-USNPs的ADSCs对伤口愈合的治疗效果，包括它们对炎症、胶原蛋白合成、血管生成和伤口闭合的影响。
在体外氧化应激条件下，用CuO-USNPs处理的ADSCs显示出活力、旁分泌活性和抗炎特性显著增强。在糖尿病小鼠中，负载CuO-USNPs的ADSCs减少了伤口组织中的炎症反应，促进了胶原蛋白合成和血管生成，并加速了糖尿病伤口愈合。这些发现表明，CuO-USNPs有效地减轻了氧化应激和炎症的不利影响，提高了ADSCs的治疗效果。
本研究提出了一种简单有效的方法来提高干细胞疗法对糖尿病伤口的治疗潜力。通过掺入CuO-USNPs，ADSCs的抗氧化和抗炎能力显著增强，为与ROS相关的组织修复和慢性伤口愈合提供了一种有前景的策略。
