Tian Yongzhi, Ma Xiaolin, Li Yuanting, Cheng Cong, An Dengdi, Ge Fengwei
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi 830054, Xinjiang, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
Biosci Rep. 2021 Apr 30;41(4). doi: 10.1042/BSR20201679.
Rhizosheath is an adaptive feature for the survival of Stipagrostis pennata in desert systems. Although microorganisms play important ecological roles in promoting the nitrogen cycle of rhizosheath, the diversity and function of nitrogen-fixing microorganism communities have not been fully understood.
Therefore, the aim of the present study is to explore the nitrogen fixation ability of rhizosheaths and the changes in abundance of nitrogen-fixing microorganisms at different growth periods of S. pennata. We sequenced the nifH gene through sequencing to identify the structure and diversity of nitrogen-fixing microorganisms of S. pennata at different growth periods of rhizosheaths.
A total of 1256 operational taxonomic units (OTUs) were identified by nifH sequence and distributed in different growth periods. There were five OTUs distributed in each sample at the same time, and the abundance and diversity of microorganisms in fruit period were much higher than those in other periods. Mainly four phyla were involved, among which Proteobacteria was the most abundant in all groups.
In general, the present study investigated the abundance and characteristics of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of S. pennata. It also may elucidate and indicate that the structure of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of S. pennata had changed.
根鞘是羽毛针禾在沙漠系统中生存的一种适应性特征。尽管微生物在促进根鞘氮循环中发挥着重要的生态作用，但固氮微生物群落的多样性和功能尚未得到充分了解。
因此，本研究的目的是探讨根鞘的固氮能力以及羽毛针禾不同生长时期固氮微生物丰度的变化。我们通过测序对nifH基因进行测序，以确定羽毛针禾根鞘不同生长时期固氮微生物的结构和多样性。
通过nifH序列共鉴定出1256个可操作分类单元（OTU），并分布于不同生长时期。每个样本同时有5个OTU分布，结果期微生物的丰度和多样性远高于其他时期。主要涉及4个门，其中变形菌门在所有组中最为丰富。
总体而言，本研究调查了羽毛针禾不同生长时期根鞘固氮微生物的丰度和特征。它还可能阐明并表明羽毛针禾不同生长时期根鞘固氮微生物的结构发生了变化。
