Salimian Rizi Sara, Rezayatmand Zahra, Ranjbar Monireh, Yazdanpanahi Nasrin, Emami-Karvani Zarrin Dokht
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Department of Biotechnology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Iran J Biotechnol. 2024 Jan 1;22(1):e3687. doi: 10.30498/ijb.2024.394178.3687. eCollection 2024 Jan.
Soil salinity is a major problem in the world that affects the growth and yield of plants. Application of new and up-to-date techniques can help plants in dealing with salinity stress. One of the approaches for reducing environmental stress is the use of rhizosphere bacteria.
The aim of present study was to investigate the effect of the inoculation of Bacillus cereus on physiological and biochemical indicators and the expression of some key genes involved in the Artemisinin biosynthesis pathway in Artemisia absinthium under salinity stress.
The study was conducted using three different salinity levels (0, 75, 150 mM/NaCl) and two different bacterial treatments (i. e, without bacterial inoculation and co-inoculation with B. cereus isolates). The data from the experiments were analyzed using factorial analysis, and the resulting interaction effects were subsequently examined and discussed.
The results showed that with increasing salinity, root and stem length, root and stem weight, root and stem dry weight, and potassium content were decreased, although the content of sodium was increased. Rhizosphere bacteria increased the contents of Artemisinin, potassium, calcium, magnesium, and iron and the expression of Amorpha-4,11-diene synthase and Cytochrome P450 monooxygenase1 genes as well as the growth indicators; although decreased the sodium content. The highest ADS expression was related to co-inoculation with B. cereus isolates E and B in 150 mM salinity. The highest CYP71AV1 expression was related to co-inoculation with B. cereus isolates E and B in 150 mM salinity.
These findings showed that the increase in growth indices under salinity stress was probably due to the improvement of nutrient absorption conditions as a result of ion homeostasis, sodium ion reduction and Artemisinin production conditions by rhizosphere B. cereus isolates E and B.
土壤盐渍化是全球范围内影响植物生长和产量的主要问题。应用新的和最新的技术可以帮助植物应对盐胁迫。减少环境胁迫的方法之一是利用根际细菌。
本研究旨在探讨盐胁迫下接种蜡样芽孢杆菌对苦艾生理生化指标以及青蒿素生物合成途径中一些关键基因表达的影响。
本研究采用三种不同的盐度水平（0、75、150 mM/NaCl）和两种不同的细菌处理（即不接种细菌和与蜡样芽孢杆菌分离株共接种）。实验数据采用析因分析进行分析，随后对产生的交互作用进行检验和讨论。
结果表明，随着盐度增加，根和茎的长度、根和茎的重量、根和茎的干重以及钾含量均降低，而钠含量增加。根际细菌增加了青蒿素、钾、钙、镁和铁的含量以及紫穗槐-4,11-二烯合酶和细胞色素P450单加氧酶1基因的表达以及生长指标；尽管降低了钠含量。在150 mM盐度下，最高的ADS表达与蜡样芽孢杆菌分离株E和B共接种有关。在150 mM盐度下，最高的CYP71AV1表达与蜡样芽孢杆菌分离株E和B共接种有关。
这些发现表明，盐胁迫下生长指标的增加可能是由于根际蜡样芽孢杆菌分离株E和B通过离子稳态、钠离子减少和青蒿素生产条件改善了养分吸收条件。
