Department of Mechanical Engineering, Abdullah Gül University, 38080, Kayseri, Turkey.
J Mater Sci Mater Med. 2021 Mar 29;32(4):35. doi: 10.1007/s10856-021-06503-z.
Cancer cells' ability to sense their microenvironment and interpret these signals for the regulation of directional adhesion plays crucial role in cancer invasion. Furthermore, given the established influence of mechanical properties of the substrate on cell behavior, the present study aims to elucidate the relationship between the contact guidance of glioblastoma cell (GBM) and evolution of microstructural and mechanical properties of the implants. SEM analyses of the specimens subjected to 5 and 25% of plastic strains revealed directional groove-like structures in micro and submicro-sizes, respectively. Microscale cytoplasmic protrusions of GBMs showed elongation favored along the grooves created via deformation markings on 5% deformed sample. Whereas filopodia, submicro-sized protrusions facilitating cancer invasion, elongated in the direction perpendicular to the deformation markings on the 25% deformed sample, which might lead to easy and rapid retraction. Furthermore, number of cell attachment was 1.7-fold greater on 25% deformed sample, where these cells showed the greatest cellular aspect ratio. The directional attachment and contact guidance of GBMs was reported for the first time on metallic implants and these findings propose the idea that GBM response could be regulated by controlling the spacing of the deformation markings, namely the degree of plastic deformation. These findings can be applied in the design of cell-instructive implants for therapeutic purposes to suppress cancer dissemination.
癌细胞感知其微环境并解释这些信号以调节定向黏附的能力在癌症侵袭中起着至关重要的作用。此外，鉴于基底的机械性能对细胞行为的已有影响，本研究旨在阐明神经胶质瘤细胞（GBM）的接触导向与植入物的微观结构和机械性能演变之间的关系。对经受 5%和 25%塑性应变的试样进行 SEM 分析，分别揭示了微和亚微观尺寸上的定向槽状结构。GBM 的微尺度细胞质突起显示出在 5%变形样品上的变形标记形成的沟槽中沿伸长方向的伸长。而丝状伪足，即促进癌症侵袭的亚微观突起，在 25%变形样品上沿与变形标记垂直的方向伸长，这可能导致易于和快速缩回。此外，在 25%变形的样品上细胞附着数量增加了 1.7 倍，这些细胞显示出最大的细胞纵横比。GBM 在金属植入物上的定向附着和接触导向被首次报道，这些发现提出了一种观点，即可以通过控制变形标记的间距（即塑性变形程度）来调节 GBM 的反应。这些发现可应用于治疗性抑制癌症扩散的细胞指令性植入物的设计中。
