Gui N, Xu W, Abraham A N, Shukla R, Qian M
Centre for Additive Manufacturing, School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia.
School of Engineering, Deakin University, Waurn Ponds, Victoria 3216, Australia.
ACS Appl Bio Mater. 2019 Jun 17;2(6):2405-2413. doi: 10.1021/acsabm.9b00094. Epub 2019 May 14.
Statistical data have consistently shown that implant loosening is a significant causative factor for revision surgeries. Both and studies have confirmed the positive influences of microgrooved titanium implant surfaces on improving orthopedic titanium implants compared with a smooth titanium surface. Complete cell-groove adhesion is a prerequisite for rapid and robust osseointegration. For the first time, this work has quantified the influence of the titanium groove width at the subcellular scale (5-20 μm) on osteoblast responses, using titanium-coated microgrooved silicon wafer specimens (surface roughness, = ∼1.5 nm), which can avoid the latent influence of variations in surface roughness from the use of normal titanium substrates. The cell-groove adhesion increased from 53.07% to 98.55% with an increasing groove width from 5 to 20 μm. In addition, both the cell spreading area and cell width were proportional to groove width. However, no statistically significant influence ( > 0.05) of groove width was identified on cell proliferation and differentiation. An exponential model was proposed to predict the groove geometries that can facilitate complete cell-groove adhesion. The underlying mechanisms were discussed. The experimental findings of this study provide a unique basis for the design of titanium implant surfaces.
统计数据一直表明，植入物松动是翻修手术的一个重要致病因素。多项研究均证实，与光滑钛表面相比，微槽钛植入物表面对改善骨科钛植入物具有积极影响。完整的细胞与凹槽黏附是快速而稳固的骨整合的先决条件。本研究首次在亚细胞尺度（5 - 20μm）上量化了钛凹槽宽度对成骨细胞反应的影响，使用的是涂钛微槽硅片标本（表面粗糙度，约为1.5nm），这可以避免使用普通钛基板时表面粗糙度变化的潜在影响。随着凹槽宽度从5μm增加到20μm，细胞与凹槽的黏附率从53.07%增加到98.55%。此外，细胞铺展面积和细胞宽度均与凹槽宽度成正比。然而，未发现凹槽宽度对细胞增殖和分化有统计学显著影响（P > 0.05）。提出了一个指数模型来预测能够促进细胞与凹槽完全黏附的凹槽几何形状，并对潜在机制进行了讨论。本研究的实验结果为钛植入物表面的设计提供了独特的依据。
