R&D Center, Cell Biotech, Co., Ltd., 50, Aegibong-ro 409 beon-gil, Gaegok-ri, Wolgot-myeon, Gimpo-si, 10003, Republic of Korea.
J Microbiol. 2021 Apr;59(4):417-425. doi: 10.1007/s12275-021-0573-2. Epub 2021 Mar 29.
Probiotics are known to protect against liver damage induced by the alcohol and acetaldehyde accumulation associated with alcohol intake. However, there have been few studies of the direct effect of probiotics on alcohol metabolism, and the types of probiotics that were previously analyzed were few in number. Here, we investigated the effects of 19 probiotic species on alcohol and acetaldehyde metabolism. Four probiotic species that had a relatively high tolerance to alcohol and metabolized alcohol and acetaldehyde effectively were identified: Lactobacillus gasseri CBT LGA1, Lactobacillus casei CBT LC5, Bifidobacterium lactis CBT BL3, and Bifidobacterium breve CBT BR3. These species also demonstrated high mRNA expression of alcohol and acetaldehyde dehydrogenases. ProAP4, a mixture of these four probiotics species and excipient, was then administered to rats for 2 weeks in advance of acute alcohol administration. The serum alcohol and acetaldehyde concentrations were significantly lower in the ProAP4-administered group than in the control and excipient groups. Thus, the administration of ProAP4, containing four probiotic species, quickly lowers blood alcohol and acetaldehyde concentrations in an alcohol and acetaldehyde dehydrogenasedependent manner. Furthermore, the serum alanine aminotransferase activity, which is indicative of liver damage, was significantly lower in the ProAP4 group than in the control group. The present findings suggest that ProAP4 may be an effective means of limiting alcohol-induced liver damage.
益生菌已知可防止与酒精摄入相关的酒精和乙醛积累引起的肝损伤。然而，对益生菌对酒精代谢的直接影响的研究很少，并且之前分析的益生菌类型数量很少。在这里，我们研究了 19 种益生菌对酒精和乙醛代谢的影响。确定了四种对酒精具有相对较高耐受性且有效代谢酒精和乙醛的益生菌：Lactobacillus gasseri CBT LGA1、Lactobacillus casei CBT LC5、Bifidobacterium lactis CBT BL3 和 Bifidobacterium breve CBT BR3。这些物种还表现出高的酒精和乙醛脱氢酶的 mRNA 表达。然后将含有这四种益生菌和赋形剂的 ProAP4 提前 2 周给予大鼠，然后给予急性酒精处理。ProAP4 给药组的血清酒精和乙醛浓度明显低于对照组和赋形剂组。因此，含有四种益生菌的 ProAP4 的给药可迅速降低血液中的酒精和乙醛浓度，这是一种依赖于酒精和乙醛脱氢酶的方式。此外，ProAP4 组的血清丙氨酸氨基转移酶活性（指示肝损伤）明显低于对照组。这些发现表明 ProAP4 可能是限制酒精性肝损伤的有效手段。
