Jung Su-Jin, Hwang Ji-Hyun, Park Eun-Ock, Lee Seung-Ok, Chung Yun-Jo, Chung Myung-Jun, Lim Sanghyun, Lim Tae-Joong, Ha Yunhi, Park Byung-Hyun, Chae Soo-Wan
Clinical Trial Center for Functional Foods, Jeonbuk National University Hospital, Jeonju, Jeonbuk 54907, Korea.
Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, Jeonbuk 54907, Korea.
Nutrients. 2021 May 30;13(6):1875. doi: 10.3390/nu13061875.
Excessive alcohol consumption is one of the most significant causes of morbidity and mortality worldwide. Alcohol is oxidized to toxic and carcinogenic acetaldehyde by alcohol dehydrogenase (ADH) and further oxidized to a non-toxic acetate by aldehyde dehydrogenase (ALDH). There are two major ALDH isoforms, cytosolic and mitochondrial, encoded by and genes, respectively. The polymorphism is associated with flushing response to alcohol use. Emerging evidence shows that and species encode alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) mediate alcohol and acetaldehyde metabolism, respectively. A randomized, double-blind, placebo-controlled crossover clinical trial was designed to study the effects of and in humans and assessed their effects on alcohol and acetaldehyde metabolism. Here, twenty-seven wild types () and the same number of heterozygotes () were recruited for the study. The enrolled participants were randomly divided into either the probiotic (Duolac ProAP4) or the placebo group. Each group received a probiotic or placebo capsule for 15 days with subsequent crossover. Primary outcomes were measurement of alcohol and acetaldehyde in the blood after the alcohol intake. Blood levels of alcohol and acetaldehyde were significantly downregulated by probiotic supplementation in subjects with genotype, but not in those with genotype. However, there were no marked improvements in hangover score parameters between test and placebo groups. No clinically significant changes were observed in safety parameters. These results suggest that Duolac ProAP4 has a potential to downregulate the alcohol and acetaldehyde concentrations, and their effects depend on the presence or absence of polymorphism on the gene.
过量饮酒是全球发病和死亡的最重要原因之一。酒精通过乙醇脱氢酶（ADH）氧化为有毒和致癌的乙醛，再通过乙醛脱氢酶（ALDH）进一步氧化为无毒的乙酸盐。有两种主要的ALDH亚型，即胞质型和线粒体型，分别由 和 基因编码。 多态性与饮酒后的脸红反应有关。新出现的证据表明， 和 物种分别编码乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH），介导酒精和乙醛代谢。一项随机、双盲、安慰剂对照的交叉临床试验旨在研究 和 对人类的影响，并评估它们对酒精和乙醛代谢的作用。在此，招募了27名野生型（ ）和相同数量的杂合子（ ）进行研究。入选的参与者被随机分为益生菌（Duolac ProAP4）组或安慰剂组。每组接受益生菌或安慰剂胶囊15天，随后进行交叉。主要结局是饮酒后血液中酒精和乙醛的测量。在 基因型的受试者中，补充益生菌可显著下调血液中酒精和乙醛的水平，但在 基因型的受试者中则不然。然而，试验组和安慰剂组之间的宿醉评分参数没有明显改善。在安全性参数方面未观察到具有临床意义的变化。这些结果表明，Duolac ProAP4有下调酒精和乙醛浓度的潜力，其作用取决于 基因上多态性的存在与否。
