Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, China.
Department of Bioinformatics, School of life Sciences and technology, Tongji University, Shanghai, China.
Oral Dis. 2022 Sep;28(6):1580-1590. doi: 10.1111/odi.13862. Epub 2021 May 4.
Oral lichen planus (OLP) is a chronic inflammatory disease that occurs in the oral mucosa with characteristic white striations lesions, recurrent erosions, and pains. The etiology and pathogenesis of OLP are still unclear.
We analyzed the bacterial community structure of buccal mucosa in patients with OLP and normal controls by high-throughput sequencing. Fluorescence in situ hybridization (FISH) was used to detect Prevotella melaninogenica (P. melaninogenica) in 13 OLP samples and 10 controls. The amounts of P. melaninogenica in OLP buccal mucosa and the expression of inflammatory cytokines in co-culture of mouse-derived macrophages with P. melaninogenica were detected by RT-qPCR.
The P. melaninogenica was more abundant in OLP than in healthy controls, and the differences were significant at the level of the phylum, family, genus, and species (p < .05). FISH showed that P. melaninogenica can invade the epithelium and even the lamina propria of OLP, while no invasion was found in the normal mucosa. Prevotella melaninogenica can adhere to and invade macrophages and then activate the transcription of IL-1β, IL-6, and TNF-α in NF-κB signaling pathway.
Prevotella melaninogenica may be involved in the pathogenic process of OLP, and its specific mechanism deserves further study.
口腔扁平苔藓（OLP）是一种发生在口腔黏膜的慢性炎症性疾病，具有特征性的白色条纹病变、反复糜烂和疼痛。OLP 的病因和发病机制尚不清楚。
我们通过高通量测序分析了 OLP 患者和正常对照者颊黏膜的细菌群落结构。荧光原位杂交（FISH）用于检测 13 例 OLP 样本和 10 例对照者中的普氏菌（P. melaninogenica）。通过 RT-qPCR 检测 OLP 颊黏膜中 P. melaninogenica 的含量和 P. melaninogenica 与鼠源性巨噬细胞共培养中炎症细胞因子的表达。
与健康对照组相比，OLP 中 P. melaninogenica 的丰度更高，在门、科、属和种水平上的差异均有统计学意义（p <.05）。FISH 显示 P. melaninogenica 可侵袭 OLP 的上皮甚至固有层，而在正常黏膜中未发现侵袭。普氏菌可以黏附并侵袭巨噬细胞，然后激活 NF-κB 信号通路中 IL-1β、IL-6 和 TNF-α 的转录。
普氏菌可能参与 OLP 的发病过程，其具体机制值得进一步研究。
