Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
J Agric Food Chem. 2021 Apr 7;69(13):3923-3931. doi: 10.1021/acs.jafc.1c00283. Epub 2021 Mar 29.
An increasing number of studies have suggested that PAH contamination in dairy products demands high concern. This study established an efficient determination method for the European Union 15 + 1 PAHs and four PAH derivatives in dairy samples using a QuEChERS method coupled with GC-QqQ-MS. The optimized method obtained a recovery of 63.38-109.17% with a precision of 3.82-15.62%, and the limit of detection and limit of quantification were 0.08-0.78 and 0.27-2.59 μg/kg, respectively. The validated method was then successfully applied to identify the 20 PAHs in 82 dairy samples, including 43 commercial milk samples and 39 milk powders. The total PAH concentrations ranged from 2.37 to 11.83 μg/kg, and benzo[]pyrene was only quantified in one milk and one milk powder sample at 0.35 and 0.42 μg/kg, respectively. The concentrations of PAH4 in milk samples and milk powders were not quantified (nq)-3.99 and nq-4.51 μg/kg, respectively. The results confirmed the appreciable occurrence of PAHs in dairy products, especially in infant formula. The data in this study provide a scientific basis for assessment on origin tracing, dietary exposure, and health risk of PAHs and their derivatives.
越来越多的研究表明，乳制品中多环芳烃的污染值得高度关注。本研究采用 QuEChERS 方法结合 GC-QqQ-MS 建立了一种测定乳制品中欧盟 15+1 种多环芳烃和 4 种多环芳烃衍生物的有效方法。优化后的方法回收率为 63.38-109.17%，精密度为 3.82-15.62%，检出限和定量限分别为 0.08-0.78 和 0.27-2.59μg/kg。该方法随后成功应用于鉴定 82 个乳制品样本中的 20 种多环芳烃，包括 43 个商业牛奶样本和 39 个奶粉样本。总多环芳烃浓度范围为 2.37-11.83μg/kg，仅在一个牛奶和一个奶粉样本中检测到苯并[a]芘，浓度分别为 0.35μg/kg 和 0.42μg/kg。牛奶样本和奶粉样本中多环芳烃 4 的浓度未检出（nq）-3.99μg/kg 和 nq-4.51μg/kg。研究结果证实了多环芳烃在乳制品中的存在，特别是在婴幼儿配方奶粉中。本研究的数据为评估多环芳烃及其衍生物的来源追溯、膳食暴露和健康风险提供了科学依据。
