Loy R, Gerlach J L, McEwen B S
Department of Neurobiology and Anatomy, University of Rochester Medical Center, NY 14642.
Brain Res. 1988 Apr 1;467(2):245-51. doi: 10.1016/0165-3806(88)90028-4.
This study has examined the distribution of [3H]estradiol and [1 alpha,2 alpha-3H]testosterone uptake in the hippocampal formation and entorhinal cortex of male and female rats. In both males and females, [3H]estradiol-binding neurons in Ammon's horn are located deep in stratum pyramidale and may correspond either to polymorphic interneurons or to early maturing pyramidal cells. Interneurons of strata oriens, lucidum and radiatum of Ammon's horn and of stratum moleculare of the subiculum also bind [3H]estradiol, as do basket cell interneurons in the polymorphic, infragranular layer of the dentate gyrus. While no granule cells appear to accumulate [3H]estradiol, these cells may be affected transsynaptically by gonadal steroids via their afferent contacts with the entorhinal cortex, which, of the areas examined, contains the greatest number of [3H]estradiol-binding neurons. While relatively few neurons concentrate [3H]estradiol in the hippocampal formation, these are localized to specific subpopulations, which may enhance their functional significance. Because there is no significant nuclear accumulation of [3H]-alpha-testosterone in either the entorhinal cortex or hippocampal formation, it appears that aromatase enzyme activity is not a major contributor to estrogen receptor occupancy in adult rats.
本研究检测了雄性和雌性大鼠海马结构及内嗅皮质中[3H]雌二醇和[1α,2α-3H]睾酮的摄取分布。在雄性和雌性大鼠中，海马角中的[3H]雌二醇结合神经元位于锥体层深部，可能对应于多形性中间神经元或早期成熟的锥体细胞。海马角的 Oriens 层、透明层和辐射层以及海马下托分子层的中间神经元也结合[3H]雌二醇，齿状回多形性颗粒下层的篮状细胞中间神经元也是如此。虽然似乎没有颗粒细胞积累[3H]雌二醇，但这些细胞可能通过与内嗅皮质的传入联系而受到性腺类固醇的突触后影响，在所检查的区域中，内嗅皮质含有最多的[3H]雌二醇结合神经元。虽然在海马结构中相对较少的神经元集中[3H]雌二醇，但这些神经元定位于特定的亚群，这可能增强它们的功能意义。由于在内嗅皮质或海马结构中均未观察到[3H]α-睾酮的显著核内积累，因此看来芳香化酶活性不是成年大鼠雌激素受体占据的主要贡献因素。
