Nascimento Silvia M, Salgueiro Fabiano, Menezes Mariângela, Oliveira Fernanda de Andréa, Magalhães Vinicius Chiapetta Portella, De Paula Joel Campos, Morris Steven
Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, 22.290-240 Rio de Janeiro, RJ, Brazil.
Grupo de Pesquisa em Biodiversidade Molecular Vegetal, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, 22.290-240 Rio de Janeiro, RJ, Brazil.
Harmful Algae. 2016 Jul;57(Pt A):39-48. doi: 10.1016/j.hal.2016.05.006. Epub 2016 Jun 10.
Morphological descriptions using light and scanning electron microscopy and molecular characterization of two Prorocentrum lima strains (UNR-01 and UNR-09) isolated from Armação dos Búzios, Rio de Janeiro, Brazil are provided. Okadaic acid (OA), dinophysistoxin-1 (DTX1) and DTX2 production by strain UNR-01 was investigated using liquid chromatography with mass spectrometry. Toxins were extracted from heat-treated (boiled) and non-boiled cell pellets to obtain respective quantities of free and total OA and DTX1. Growth parameters (growth rate and mean generation time) were determined for strain UNR-01. Prorocentrum lima cells were oblong-to-ovate in shape, broad in the middle region, and narrow at the anterior end. The periflagellar area was triangular, set into a V-shaped depression and was composed of eight periflagellar platelets of different sizes. The morphology fits well the characterization of the species isolated from elsewhere. Phylogenetic analysis based on internal transcribed spacer - ITS - and D1-D3 large subunit - LSU - of ribosomal RNA gene sequences revealed that both strains were identical and closely related to P. lima isolates from the Caribbean Sea and USA. The growth rate of strain UNR-01 was 0.24divday. OA concentrations were on average 15.2 and 38.5pg[OA]cell for heat-treated and non-treated cells respectively, while DTX1 mean concentration was 0.5pg[DTX1]cell for both heat-treated and non-treated cells. DTX2 was not detected. To date, these are the first strains of P. lima from the south Atlantic that have been characterized.
本文提供了对从巴西里约热内卢布齐奥斯湾分离出的两株利马原甲藻（Prorocentrum lima）（UNR - 01和UNR - 09）的形态学描述，包括使用光学显微镜和扫描电子显微镜进行的观察，以及分子特征分析。使用液相色谱 - 质谱联用技术研究了菌株UNR - 01产生的冈田酸（OA）、鳍藻毒素 - 1（DTX1）和DTX2。从热处理（煮沸）和未煮沸的细胞沉淀中提取毒素，以分别获得游离和总OA及DTX1的含量。测定了菌株UNR - 01的生长参数（生长速率和平均世代时间）。利马原甲藻细胞呈长椭圆形至卵形，中部宽阔，前端狭窄。鞭毛周区域呈三角形，呈V形凹陷，由八个不同大小的鞭毛周血小板组成。该形态与从其他地方分离出的该物种特征非常吻合。基于核糖体RNA基因序列的内部转录间隔区（ITS）和D1 - D3大亚基（LSU）的系统发育分析表明，这两株菌株相同，且与来自加勒比海和美国的利马原甲藻分离株密切相关。菌株UNR - 01的生长速率为0.24次/天。热处理和未处理细胞的OA浓度分别平均为15.2和38.5 pg/[OA]细胞，而热处理和未处理细胞的DTX1平均浓度均为0.5 pg/[DTX1]细胞。未检测到DTX2。迄今为止，可以确定这是来自南大西洋的首批已被鉴定的利马原甲藻菌株。
