School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Arch Microbiol. 2021 Aug;203(6):3055-3059. doi: 10.1007/s00203-021-02294-7. Epub 2021 Mar 30.
A novel actinomycete strain, designated strain QMT-12, was isolated from the rhizospheric soils of Fagopyrum tataricum and characterized using a polyphasic approach. Strain QMT-12 was found to have morphological features typical of the genus Streptomyces. The predominant fatty acids included C cis9 (35.9%), Summed feature 6 (C cis9, 12/C a or C anteiso/C c) (30.6%) and C (16.3%). The whole-cell sugars were arabinose and glucose. The whole-cell-wall amino acids included alanine, aspartate, glutamic acid, glycine and LL-diaminopimelic acid. The menaquinones were MK-9, MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The diagnostic phospholipids consisted of diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidyl methyl ethanolamine, phospholipids, phosphotidyl inositol, phosphotidylinositol mannosides, and phospholipids of unknown structure containing glucosamine. The full-length 16S rRNA gene sequence analysis showed that strain QMT-12 belonged to the genus Streptomyces and had 98.2, 98.1, 98.1 and ≤ 98.0% similarities to Streptomyces camponoticapitis 2H-TWYE14, Streptomyces scopuliridis NRRL B-24574, Streptomyces inhibens NEAU-D10 and other Streptomyces species with validly published and correct names, respectively. Phylogenetic analysis indicated that strain QMT-12 was closely related to Streptomyces inhibens NEAU-D10. However, the average nucleotide identity value and the digital DNA-DNA hybridization value between strain QMT-12 and S. inhibens NEAU-D10 were 85.0 and 22.3%, respectively, well below 95-96% and 70% cut-off point recommended for delineating species. Based on its phenotypic and genotypic characteristics, strain QMT-12 (= CICC 11056 = JCM 33963) represents the type strain of a novel species, for which the name Streptomyces liangshanensis sp. nov. is proposed.
一株新的放线菌菌株，命名为 QMT-12 菌株，从苦荞麦的根际土壤中分离得到，并采用多相分类法进行了特征描述。QMT-12 菌株具有链霉菌属的典型形态特征。主要脂肪酸包括顺式 9（35.9%）、Summed feature 6（顺式 9,12/C a 或 C anteiso/C c）（30.6%）和 C 16.3%。细胞壁糖为阿拉伯糖和葡萄糖。细胞壁氨基酸包括丙氨酸、天冬氨酸、谷氨酸、甘氨酸和 LL-二氨基庚二酸。主要的醌为 MK-9、MK-9(H)、MK-9(H)、MK-9(H)和 MK-9(H)。鉴定的磷脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和含有葡萄糖胺的未知结构的磷脂。全 16S rRNA 基因序列分析表明，QMT-12 菌株属于链霉菌属，与 Camponoticapitis 2H-TWYE14 链霉菌、Scopuliridis NRRL B-24574 链霉菌、Inhibens NEAU-D10 链霉菌和其他具有有效发表和正确名称的链霉菌种的相似度分别为 98.2、98.1、98.1 和≤98.0%。系统发育分析表明，QMT-12 菌株与 Inhibens NEAU-D10 链霉菌关系密切。然而，QMT-12 菌株与 Inhibens NEAU-D10 链霉菌之间的平均核苷酸同一性值和数字 DNA-DNA 杂交值分别为 85.0%和 22.3%，均远低于 95-96%和 70%的种界定推荐值。基于表型和基因型特征，QMT-12 菌株（=CICC 11056=JCM 33963）代表一个新种的模式菌株，提议将其命名为梁山河链霉菌（Streptomyces liangshanensis sp. nov.）。
