对分箱或时间选通的周期性衰变进行连续和离散相量分析。

Department of Chemistry and Biochemistry, 607 Charles E. Young Drive E., Los Angeles, California 90095, USA.

AIP Adv. 2021 Mar 23;11(3):035331. doi: 10.1063/5.0027834. eCollection 2021 Mar.

The time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of instrument response function offset, decay truncation, and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented, and an illustration of the new concepts with examples from the literature concludes this work.

本文重新探讨了在存在时间选通或分箱的情况下，通过相量法对周期性激发发光衰减进行时间分辨分析。推导了方形门离散配置的解析表达式，并将这种修正的周期性单指数衰减的相量轨迹与标准通用半圆进行了比较。还讨论了仪器响应函数偏移、衰减截断和门形状的影响。最后，针对一些简单情况给出了相位和模量寿命的修正表达式。本文还讨论了一种修正的相量校准方法，并通过文献中的示例对新概念进行了说明，以此结束本文工作。