使用新型时间选通单光子雪崩二极管相机进行近红外荧光寿命宏观成像。
NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.
作者信息
Michalet Xavier, Ulku Arin C, Wayne Michael A, Weiss Shimon, Bruschini Claudio, Charbon Edoardo
机构信息
Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA 90095.
AQUA Lab, Ecole polytechnique fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.
出版信息
Proc SPIE Int Soc Opt Eng. 2023 Jan-Feb;12384. doi: 10.1117/12.2649227. Epub 2023 Apr 25.
SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.
SwissSPAD3是为荧光寿命成像（FLI）应用而开发的宽视场时间选通单光子雪崩二极管（SPAD）成像仪系列中的最新产品。其独特之处在于：（i）能够定义比其前代产品更短的选通门（宽度<1 ns）；（ii）支持高达至少80 MHz的激光重复频率；（iii）采用双选通门架构，有效占空比为100%。我们展示了使用相量法分析的短寿命近红外染料的宽视场宏观FLI测量结果。将结果与之前使用SwissSPAD2获得的结果以及理论预测进行了比较。
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