Michalet Xavier, Ulku Arin C, Wayne Michael A, Weiss Shimon, Bruschini Claudio, Charbon Edoardo
Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA 90095.
AQUA Lab, Ecole polytechnique fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.
Proc SPIE Int Soc Opt Eng. 2023 Jan-Feb;12384. doi: 10.1117/12.2649227. Epub 2023 Apr 25.
SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.
SwissSPAD3是为荧光寿命成像（FLI）应用而开发的宽视场时间选通单光子雪崩二极管（SPAD）成像仪系列中的最新产品。其独特之处在于：（i）能够定义比其前代产品更短的选通门（宽度<1 ns）；（ii）支持高达至少80 MHz的激光重复频率；（iii）采用双选通门架构，有效占空比为100%。我们展示了使用相量法分析的短寿命近红外染料的宽视场宏观FLI测量结果。将结果与之前使用SwissSPAD2获得的结果以及理论预测进行了比较。
