Li Shaoguang, Wang Yuanyuan, Zhang Zishuo, Wang Yiming, Li Hui, Xia Fan
Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
Anal Chem. 2021 Apr 13;93(14):5849-5855. doi: 10.1021/acs.analchem.1c00085. Epub 2021 Mar 31.
The continuous, real-time monitoring of specific analytes in situ in biological fluids would provide personalized, high-precision pharmacokinetic information for the goal of precision medicine. Due to their conformationally linked signaling mechanism, electrochemical aptamer-based (E-AB) sensors are promising candidates for accurate measurements in such complex media. They suffer, however, from severe baseline drift when interrogated continuously and in real-time manner. In response, here, we investigate a couple of self-assembled monolayers in the application of E-AB sensors, achieving the improvement of their baseline stability and simultaneous modulation of sensor performance, e.g., target affinity and specificity.
对生物流体中的特定分析物进行原位连续实时监测，将为精准医疗目标提供个性化、高精度的药代动力学信息。基于电化学适配体（E-AB）的传感器由于其构象连接的信号传导机制，有望在这种复杂介质中进行准确测量。然而，当以连续和实时方式进行检测时，它们会出现严重的基线漂移。对此，我们在此研究了几种自组装单分子层在E-AB传感器中的应用，实现了基线稳定性的改善以及传感器性能（如目标亲和力和特异性）的同步调节。
