Shaver Alexander, Arroyo-Currás Netzahualcóyotl
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Curr Opin Electrochem. 2022 Apr;32. doi: 10.1016/j.coelec.2021.100902. Epub 2021 Dec 1.
Nucleic acid-based electrochemical sensors are a versatile technology enabling affinity-based detection of a great variety of molecular targets, regardless of inherent electrochemical activity or enzymatic reactivity. Additionally, their modular interface and ease of fabrication enable rapid prototyping and sensor development. However, the technology has inhibiting limitations in terms of long-term stability that have precluded translation into clinically valuable platforms like continuous molecular monitors. In this opinion, we discuss published methods to address various aspects of sensor stability, including thiol-based monolayers and anti-biofouling capabilities. We hope the highlighted works will motivate the field to develop innovative strategies for extending the long-term operational life of nucleic acid-based electrochemical sensors.
基于核酸的电化学传感器是一种通用技术，能够对多种分子靶标进行基于亲和力的检测，而无需考虑其固有的电化学活性或酶促反应活性。此外，其模块化接口和易于制造的特点使得能够快速进行原型设计和传感器开发。然而，该技术在长期稳定性方面存在限制，这阻碍了其转化为像连续分子监测仪这样具有临床价值的平台。在本观点文章中，我们讨论了已发表的解决传感器稳定性各个方面的方法，包括基于硫醇的单分子层和抗生物污损能力。我们希望这些突出的研究成果能够激励该领域开发创新策略，以延长基于核酸的电化学传感器的长期使用寿命。
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