Laboratory of Environmental Health, Laboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
Department of Botany, University of Dhaka, Dhaka, Bangladesh.
J Appl Microbiol. 2021 Nov;131(5):2539-2551. doi: 10.1111/jam.15093. Epub 2021 Apr 15.
To determine the occurrence, diversity, antibiotic resistance and biofilm formation of Vibrio parahaemolyticus isolated from marine fishes in Bangladesh.
A total of 80 marine fishes were obtained from the local markets and examined for the presence of V. parahaemolyticus. All the isolated V. parahaemolyticus were characterized for the presence of virulence markers, thermostable direct hemolysin (TDH) or thermostable direct hemolysin related hemolysin (TRH). Isolates were serotyped and further characterized by enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) typing to analyse the genetic diversity. Moreover, biofilm formation and antibiotic resistance patterns were also determined. About 63·75% (51/80) of the tested marine fishes were contaminated with V. parahaemolyticus. From the contaminated fishes, 71 representatives V. parahaemolyticus were isolated and none of them harboured tdh and trh virulence genes. Nine different O-groups and seven different K-types were found by serological analysis and the dominant serotype was O5:KUT. In ERIC-PCR analysis, eight clusters (A-H) were found and the most common pattern was A (46·5%). All of the isolates were resistant to ampicillin and 78·9% of isolates were resistant to streptomycin. The highest biofilm formation was found at 37°C compared to 25°C and 4°C.
Diverse V. parahaemolyticus are present in marine fishes in the local market of Bangladesh with antibiotic-resistant properties and biofilm formation capacity.
The widespread prevalence of diverse V. parahaemolyticus in marine fishes is an issue of serious concern, and it entails careful monitoring to ascertain the safety of seafood consumers.
确定从孟加拉国海洋鱼类中分离的副溶血性弧菌的发生、多样性、抗生素耐药性和生物膜形成。
从当地市场获得 80 条海洋鱼类，以确定副溶血性弧菌的存在。所有分离的副溶血性弧菌均进行了毒力标记物耐热直接溶血素（TDH）或耐热直接溶血素相关溶血素（TRH）的检测。对分离株进行血清型分析，并进一步通过肠细菌重复基因间一致性聚合酶链反应（ERIC-PCR）分型进行基因多样性分析。此外，还确定了生物膜形成和抗生素耐药模式。约 63.75%（51/80）的测试海洋鱼类受到副溶血性弧菌的污染。从污染的鱼类中分离出 71 株代表副溶血性弧菌，它们均未携带 tdh 和 trh 毒力基因。血清学分析发现 9 个不同的 O 群和 7 个不同的 K 型，优势血清型为 O5:KUT。在 ERIC-PCR 分析中，发现了 8 个聚类（A-H），最常见的模式是 A（46.5%）。所有分离株均对氨苄西林耐药，78.9%的分离株对链霉素耐药。与 25°C 和 4°C 相比，在 37°C 时生物膜形成最高。
在孟加拉国当地市场的海洋鱼类中存在具有抗生素耐药性和生物膜形成能力的多种副溶血性弧菌。
在海洋鱼类中广泛存在的多种副溶血性弧菌是一个严重关切的问题，需要进行仔细监测，以确保海鲜消费者的安全。
