Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India.
Mikrochim Acta. 2021 Apr 1;188(4):145. doi: 10.1007/s00604-021-04751-y.
Vitamin D (VD) deficiency is a global health concern due to its serious health impacts, and at present, the monitoring of VD status is expensive. Here, a novel immunosensor for sensitive and label-free detection of 25-hydroxy vitamin D (25VD) is reported. Nanostructured cerium(IV) oxide (nCeO) was anchored onto carbon cloth (CC) via electrophoretic deposition to fabricate a nanoplatform (nCeO/CC). Subsequently, bioactive molecules (anti-25VD and BSA) were introduced to fabricate the nanobioplatform BSA/anti-25VD/nCeO/CC as an immunosensor. The analytical performance of the developed immunosensor was studied towards 25VD detection. The immunosensor provides a broad linear range of 1-200 ng mL, high sensitivity of 2.08 μA ng mL cm, a detection limit of 4.63 ng mL, and a response time of 15 min, which is better than that of previous reports. The biosensor exhibited high selectivity, good reproducibility, and excellent stability for about 45 days. The potential application of the proposed immunosensor was observed for real serum samples towards 25VD detection that demonstrated a high correlation with the conventional enzyme-linked immunosorbent assay. Graphical abstract.
维生素 D (VD) 缺乏是一个全球性的健康问题，因为它会对健康造成严重影响，而目前，VD 状况的监测费用昂贵。在这里，我们报道了一种用于敏感和无标记检测 25-羟维生素 D (25VD) 的新型免疫传感器。通过电泳沉积将纳米结构的氧化铈 (nCeO) 锚定在碳纤维布 (CC) 上，以制备纳米平台 (nCeO/CC)。随后，将生物活性分子 (抗 25VD 和 BSA) 引入以制备纳米生物平台 BSA/抗 25VD/nCeO/CC 作为免疫传感器。研究了所开发的免疫传感器对 25VD 检测的分析性能。该免疫传感器在 1-200ngmL 的宽线性范围内提供了高灵敏度，为 2.08 μA ng mL cm，检测限为 4.63ng mL，响应时间为 15min，优于以往的报道。该生物传感器表现出高选择性、良好的重现性和出色的稳定性，约 45 天。该免疫传感器在实际血清样本中对 25VD 的检测表现出了高相关性，与传统的酶联免疫吸附测定法一致。
