School of Pharmaceutical Technology, Adamas University, Barasat, Kolkata, India.
Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, India.
J Pharm Pharmacol. 2021 Mar 8;73(4):560-572. doi: 10.1093/jpp/rgaa071.
The study was carried out to evaluate the in vivo antiurolithic efficaciousness of an ethyl acetate fraction of Aerva lanata (EAFAL) derived from the hydromethanolic extract of its aerial parts (HMEAL).
In vivo pharmacological potency of EAFAL was assessed by ethylene glycol (EG) induced urolithiasis model in male Wistar albino rats. Urine samples of the animals were analysed for physical parameters, stone promoters, inhibitors along with an evaluation of the biochemical parameters of serum and kidneys. Histopathological investigation of the kidneys was done. The fraction was further subjected to LC-MS and HPLC for its phytochemical evaluation.
EAFAL demonstrated a significant antiurolithic effect by a restoration of the balance between urinary promoters and inhibitors along with an amelioration of the urinary pH. The abnormally elevated levels of serum nitrogenous substances, calcium, albumin, globulin, total protein along with altered renal calcium, oxalate and uric acid were also alleviated significantly followed by an improvement of the histopathological aberrancies. Phytochemical analysis showed evidence of phenolic components and flavonoids.
The current findings prove the beneficial role of phenolic and flavonoid rich EAFAL in ameliorating urolithiasis induced abnormalities of urine, serum and kidneys.
本研究旨在评估从其地上部分水甲醇提取物中获得的 Aerva lanata 的乙酸乙酯级分（EAFAL）的体内抗结石功效。
通过雄性 Wistar 白化大鼠乙二醇（EG）诱导的尿石症模型评估 EAFAL 的体内药理功效。对动物的尿液样本进行物理参数、结石促进剂和抑制剂分析，以及对血清和肾脏的生化参数进行评估。对肾脏进行组织病理学检查。对该级分进行进一步的 LC-MS 和 HPLC 分析，以评估其植物化学成分。
EAFAL 通过恢复尿促进剂和抑制剂之间的平衡，以及改善尿 pH 值，显示出显著的抗结石作用。血清含氮物质、钙、白蛋白、球蛋白、总蛋白以及肾脏钙、草酸盐和尿酸的异常升高水平也显著降低，随后组织病理学异常得到改善。植物化学分析显示出酚类成分和类黄酮的证据。
目前的研究结果证明富含酚类和类黄酮的 EAFAL 在改善尿、血清和肾脏引起的尿石症异常方面具有有益作用。
