Sogodogo E, Doumbo O, Kouriba B, Aboudharam G
Aix Marseille Université. IRD, MEPHI, IHU-Méditerranée Infection, Marseille, France.
IHU Méditerranée Infection, Marseille, France.
New Microbes New Infect. 2021 Feb 3;40:100844. doi: 10.1016/j.nmni.2021.100844. eCollection 2021 Mar.
Different oral hygiene practices are used to overcome endemic diseases such as dental caries and oral infections. In Mali (Africa), natural plant-based toothbrushes are used for eliminating bacterial biofilm. The repertoire of microorganisms associated with natural toothbrushes is unknown. The aim of our study is to study microbial flora in particular the methanogenic archaea associated with natural toothbrushes recently recognized as responsible for periodontitis and peri-implantitis. We investigated the methanogens and bacteria associated with 15 different natural plant toothbrushes collected in Bamako local market (Mali). Microbiological investigations consisted in culturing the bacteria on agar plates and searching archaea using molecular techniques. No archaea were demonstrated by molecular biology but 50 bacterial species, including 33 aero-anaerobic and 17 aerobic species, were isolated from natural toothbrushes. We isolated sp., sp. and , which are acknowledged as opportunistic human pathogens. This study has highlighted the likely impact of the use of natural toothbrushes in the spread of potentially pathogenic bacteria in the human oral cavity.
人们采用不同的口腔卫生习惯来预防龋齿和口腔感染等地方病。在非洲的马里，人们使用天然植物牙刷来清除细菌生物膜。与天然牙刷相关的微生物种类尚不清楚。我们研究的目的是研究微生物群落，特别是与天然牙刷相关的产甲烷古菌，最近人们认识到这些古菌是导致牙周炎和种植体周围炎的原因。我们调查了从巴马科当地市场（马里）收集的15种不同天然植物牙刷上的产甲烷菌和细菌。微生物学调查包括在琼脂平板上培养细菌以及使用分子技术寻找古菌。分子生物学未检测到古菌，但从天然牙刷中分离出了50种细菌，包括33种需氧 - 厌氧菌和17种需氧菌。我们分离出了 菌属、 菌属和 菌属，这些被认为是人类机会致病菌。这项研究突出了使用天然牙刷在人类口腔中传播潜在致病细菌的可能影响。
