Gurumurthy Channabasavaiah B, Saunders Thomas L, Ohtsuka Masato
Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA.
Department of Internal Medicine, Division of Genetic Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
J Biomed Res. 2021 Mar 26;35(2):76-90. doi: 10.7555/JBR.35.20200197.
Genetically engineered mouse (GEM) models are commonly used in biomedical research. Generating GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical staff. Because of these reasons, most research institutes set up centralized core facilities where custom GEMs are created for research groups. Researchers, on the other hand, when they begin thinking about generating GEMs for their research, several questions arise in their minds. For example, what type of model(s) would be best useful for my research, how do I design them, what are the latest technologies and tools available for developing my model(s), and finally how to breed GEMs in my research. As there are several considerations and options in mouse designs, and as it is an expensive and time-consuming endeavor, careful planning upfront can ensure the highest chance of success. In this article, we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s) for their work.
基因工程小鼠（GEM）模型常用于生物医学研究。生成GEM涉及一系列复杂的实验程序，需要精密的设备和技术娴熟的技术人员。由于这些原因，大多数研究机构都设立了集中的核心设施，为研究团队创建定制的GEM。另一方面，研究人员在开始考虑为其研究生成GEM时，脑海中会出现几个问题。例如，哪种类型的模型对我的研究最有用，我该如何设计它们，有哪些可用于开发我的模型的最新技术和工具，最后如何在我的研究中培育GEM。由于小鼠设计中有多种考虑因素和选项，而且这是一项昂贵且耗时的工作，因此提前仔细规划可以确保成功的几率最高。在本文中，我们简要回答了研究人员开始考虑为其工作生成小鼠模型时出现的几个常见问题。
