Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Braz J Microbiol. 2021 Jun;52(2):1021-1027. doi: 10.1007/s42770-021-00474-7. Epub 2021 Apr 2.
Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT. In the PRNT, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT, whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.
黄病毒属病毒（WNV）、圣路易斯脑炎病毒（SLEV）、伊蚊病毒（ILHV）和罗西奥病毒（ROCV）以前在巴西的不同地区有报道，但巴西南部的研究仍然很少。为了增加巴西南部黄病毒的信息，使用 RT-qPCR 和针对 WNV 的商业 ELISA-Ab 对马血清样本进行了分析，随后进行了 PRNT。通过实时 RT-PCR 分析的 1000 个样本均为阴性。对 465 个亚样本进行了针对 WNV 的商业 ELISA-Ab 分析，其中 18.5%（86/465）的阳性样本进一步进行了 PRNT 分析。在 PRNT 中，13/86 和 2/86 匹马对 SLEV 和 WNV 呈阳性。观察到在 PRNT 中，5.8%（13/226）的农场至少有一只动物对 SLEV 呈阳性，而对 WNV 呈阳性的农场为 0.9%（2/226）。与以前在巴西其他地区报告的数据相比，我们的结果表明，公共卫生专业人员必须意识到这些潜在的人畜共患病病原体的存在。
