Appl Opt. 2021 Apr 1;60(10):B65-B80. doi: 10.1364/AO.416902.
Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies. HT uses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology. We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.
全息层析成像（HT）是一种用于生物研究的先进的无标记光学显微成像方法。HT 使用数字全息显微镜记录生物样本的复振幅作为数字全息图，然后在数值上重建样本的三维折射率（RI）分布。RI 值是无标记生物检测的关键参数，与细胞及其内部细胞器、组织和小规模生物物体的代谢活动以及生物物理参数的时空分布相关。本文深入探讨了这一快速发展的 HT 研究领域及其在生物学中的应用。我们对 HT 原理进行了综述总结，并强调了 HT 及其应用的最新技术进展。
