Appl Opt. 2021 Feb 1;60(4):A277-A284. doi: 10.1364/AO.404376.
Holographic tomography allows the 3D mapping of the refractive index of biological samples thanks to reconstruction methods based on the knowledge of illumination directions or rotation angles of the imaged sample. Recently, phase contrast tomographic flow cytometry by digital holography has been demonstrated to reconstruct the three-dimensional refractive index distribution of single cells while they are flowing along microfluidic channels. In this system, the illumination direction is fixed while the sample's rotation is not deterministically known a priori but induced by hydrodynamic forces. We propose here a technique to retrieve the rolling angles, based on a new phase images similarity metric that is capable of identifying a cell's orientations from its 3D positioning while it is flowing along the microfluidic channel. The method is experimentally tested and also validated through appropriate numerical simulations. We provide demonstration of concept by achieving reconstruction of breast cancer cells tomography.
全息层析成像技术通过基于照明方向或成像样本旋转角度知识的重建方法，允许对生物样本的折射率进行 3D 映射。最近，通过数字全息术的相衬层析流式细胞术已经证明可以重建单个细胞在沿微流控通道流动时的三维折射率分布。在该系统中，照明方向是固定的，而样本的旋转则不是先验确定的，而是由流体动力引起的。我们在这里提出了一种基于新的相位图像相似性度量的技术来恢复滚动角，该度量能够从细胞在微流控通道中流动时的三维定位中识别细胞的方向。该方法通过适当的数值模拟进行了实验验证。我们通过实现乳腺癌细胞层析成像的重建，提供了概念验证。
