School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
School of Materials Science and Engineering, Institute for Advanced Materials, Jiangsu University, Zhenjiang, Jiangsu, China.
Food Chem. 2021 Sep 1;355:129479. doi: 10.1016/j.foodchem.2021.129479. Epub 2021 Mar 6.
Applications of cyanidin-3-O-glucoside (C3G) are limited due to the poor stabilities. In this work, we proposed using silk fibroin peptide (SFP) to bind with C3G and form nanocomposites (134.73 ± 4.51 nm) for stabilization. When interacted with C3G, the fluorescence of SFP contributed by tyrosine and phenylalanine amino acids was quenched, which was proved a static quenching with the β-sheet structure of SFP unchanged. With the further exploration of the physicochemical stabilities of C3G in the nanocomposites, we demonstrated that the tolerance of C3G to the alkaline environment and the retention ratio of C3G in various concentrations of metallic ion Cu were significantly improved. In addition, the heat resistance of C3G in SFP at 80 °C was also enhanced with up to an increase of 2.5 times for the average half-life of C3G. Our results shed light on SFP could enhance physicochemical stabilities of C3G with maintaining its antioxidant activity.
由于较差的稳定性，飞燕草素-3-O-葡萄糖苷（C3G）的应用受到限制。在这项工作中，我们提出使用丝素肽（SFP）与 C3G 结合形成纳米复合材料（134.73±4.51nm）以实现稳定化。当与 C3G 相互作用时，丝素肽中由酪氨酸和苯丙氨酸氨基酸贡献的荧光被猝灭，这被证明是一种静态猝灭，丝素肽的β-折叠结构没有改变。通过进一步探索 C3G 在纳米复合材料中的物理化学稳定性，我们证明了 C3G 对碱性环境的耐受性以及在各种浓度金属离子 Cu 中的保留率显著提高。此外，在 80°C 下，C3G 在丝素肽中的耐热性也得到了增强，C3G 的平均半衰期增加了 2.5 倍。我们的结果表明，丝素肽可以提高 C3G 的物理化学稳定性，同时保持其抗氧化活性。
