Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
Stem Cell Res. 2021 Oct;56:102546. doi: 10.1016/j.scr.2021.102546. Epub 2021 Sep 20.
Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients.
胶质母细胞瘤是最常见的恶性原发性脑肿瘤，预后不良，长期生存率低。在这里，我们描述了从一名 24 岁女性胶质母细胞瘤患者的外周血单核细胞 (PBMC) 中产生的 iPSC 系。PBMC 被非整合性仙台病毒与人类 OKSM（OCT3/4、SOX2、KLF4 和 c-MYC）转录因子重编程。通过免疫细胞化学，生成的 iPSC 对多能干细胞标志物呈阳性。此外，它们还显示出正常的核型，并具有在体外自发分化为三个胚层的潜能。我们的模型可能有助于研究胶质母细胞瘤患者的发病机制。
