WALA Heilmittel GmbH, Department of Analytical Development & Research, Section Phytochemical Research, 73087 Bad Boll, Germany.
Institute of Botany, Hohenheim University, 70599 Stuttgart, Germany.
Molecules. 2021 Mar 11;26(6):1530. doi: 10.3390/molecules26061530.
In the present study, L. and L. were compared for the first time with regard to their phenolic compound profile and antioxidant activity by applying the 2,2-diphenyl-picryl hydrazyl radical assay. For this purpose, aerial plant parts were consecutively extracted with solvents of increasing polarity (dichloromethane, -butanol, ethyl acetate), revealing that the ethyl acetate fraction showed the highest antioxidant activity with an IC value of 12.2 ± 0.29 µg/mL compared to 17.0 ± 0.26 µg/mL for . Both species revealed the presence of luteolin, apigenin, centaureidin, and nevadensin exclusively in this most polar fraction, which are known as effective 2,2-diphenyl-picryl hydrazyl radical scavengers. The antioxidant capacity of the aforementioned fractions strikingly correlated with their total phenolic contents, which was highest in the ethyl acetate fraction of . Characterization of the metabolite profiles of both species showed only marginal differences in the presence of key compounds, whereas the concentrations of individual compounds appeared to be species-specific. Our results suggest that , based on its compound pattern and bioactivity characteristics, has similar qualities for phytotherapy as .
在本研究中，首次比较了 L.和 L.的酚类化合物谱和抗氧化活性，方法是应用 2,2-二苯基-1-苦基肼自由基测定法。为此，连续用极性递增的溶剂（二氯甲烷、正丁醇、乙酸乙酯）提取地上植物部分，结果表明，与 相比，乙酸乙酯部分具有最高的抗氧化活性，IC 值为 12.2±0.29 µg/mL，而 为 17.0±0.26 µg/mL。两种植物均仅在这一最极性的部分中存在木樨草素、芹菜素、山奈酚和新戊烯素，这些物质是已知的有效 2,2-二苯基-1-苦基肼自由基清除剂。上述部分的抗氧化能力与总酚含量显著相关，而 乙酸乙酯部分的总酚含量最高。两种植物代谢物图谱的特征表明，关键化合物的存在仅有微小差异，而个别化合物的浓度似乎是种特异性的。我们的结果表明，根据其化合物模式和生物活性特征，L.在植物疗法方面与 L.具有相似的品质。
