高效液相色谱分析、艾蒿和皱叶酸模的遗传毒性和抗氧化潜力

HPLC analysis, genotoxic and antioxidant potential of Achillea millefolium L. and Chaerophyllum villosum Wall ex. Dc.

机构信息

Pharmacognosy Laboratory, Department of Botany, University of Peshawar, Peshawar, Pakistan.

Centre for Plant Sciences and Biodiversity, University of Swat, Charbagh, Pakistan.

出版信息

BMC Complement Med Ther. 2024 Feb 16;24(1):91. doi: 10.1186/s12906-024-04344-1.

DOI:10.1186/s12906-024-04344-1

PMID:38365652

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10870592/

Abstract

BACKGROUND

Methanolic and chloroformic extract of Achillea millefolium and Chaerophyllum villosum were evaluated for HPLC analysis, genotoxic and antioxidant potential.

MATERIALS AND METHODS

Genotoxic activity was carried out on human blood lymphocytes via comet assay and antioxidant activity was studied through DPPH method.

RESULTS

The genotoxic potential of A. millefolium and C. villosum's methanolic and chloroformic extract was analysed using comet assay technique. Comet shaped human lymphocytes cells were observed when treated with different concentrations (50 mg/mL, 75 mg/mL, 100 mg/mL) of methanolic and chloroformic extract of both plants. Reading was taken on the basis of damaged DNA head and tail length. Greater the length of tail as compared to head, greater will be the damage and vice versa. Total comet score was obtained from A. millefolium subjected to different concentrations. After a time interval of 24 h both the extract showed dose dependant genoprotection with maximum genoprotectivity at 98.7 ± 12.7 and 116 ± 5.3 at 50 mg/100 mL for methanolic and chloroformic extract respectively. Similarly Total Comet score was obtained from C. villosum subjected to different concentrations of methanolic and chloroformic extract. After 24 h exhibited dose dependent genoprotection with maximum protectivity at 85.7 ± 22.0 and 101.7 ± 8.6 at 50 mg/100 mL for methanolic and chloroformic extract were determined. The antioxidant activity revealed that methanolic extract of A. millefolium showed highest antioxidant activity (84.21%) at 300 mg/ml after 90 min while the chloroformic extract of C. villosum exhibited highest (68.46%) antioxidant activity (59.69%) at 300 µg/ml after 90 min but less than the standard drug ascorbic acid (88.72%). Quantitative phytochemical screening revealed high percentage of alkaloids (27.4%), Phenols (34.5%), Flavonoids (32.4%) as compared to Tannins (12%) in methanolic extract of A.millefolium. While high percentage of alkaloids (31.4), Phenols (19.3%), Flavonoids (35.5%) as compared to Tannins (16.6%) in chloroformic extract of C. villosum.

CONCLUSION

The present results showed that A. millefolium and C. villosum possess a number of important compounds and revealed genoprotective property which may be used to treat several genetic disorders such as alzeimer's disease in future (Grodzicki W, Dziendzikowska K, Antioxidants 9(3):229, 2020).

摘要

背景

对春黄菊和茴芹的甲醇和氯仿提取物进行了高效液相色谱分析、遗传毒性和抗氧化潜力评估。

材料和方法

通过彗星试验研究了人类血液淋巴细胞的遗传毒性活性，通过 DPPH 法研究了抗氧化活性。

结果

使用彗星试验技术分析了春黄菊和茴芹甲醇和氯仿提取物的遗传毒性潜力。当用不同浓度（50mg/ml、75mg/ml、100mg/ml）的两种植物的甲醇和氯仿提取物处理时，观察到彗星状的人类淋巴细胞。根据受损 DNA 头和尾的长度进行读数。与头部相比，尾部越长，损伤越大，反之亦然。从不同浓度的春黄菊中获得总彗星评分。24 小时后，两种提取物均表现出剂量依赖性的遗传保护作用，甲醇提取物的最大遗传保护率分别为 98.7±12.7 和 116±5.3，氯仿提取物分别为 50mg/100ml。同样，从不同浓度的春黄菊甲醇和氯仿提取物中获得了总彗星评分。24 小时后，在 50mg/100ml 时，两种提取物均表现出剂量依赖性的遗传保护作用，最大保护率分别为 85.7±22.0 和 101.7±8.6。抗氧化活性表明，春黄菊甲醇提取物在 90 分钟时显示出最高的抗氧化活性（84.21%），在 300mg/ml 时，而茴芹氯仿提取物在 90 分钟时显示出最高的抗氧化活性（68.46%），在 300μg/ml 时，为 59.69%，但低于标准药物抗坏血酸（88.72%）。定量植物化学筛选显示，春黄菊甲醇提取物中的生物碱（27.4%）、酚类（34.5%）、类黄酮（32.4%）的百分比高于单宁（12%）。而茴芹氯仿提取物中的生物碱（31.4%）、酚类（19.3%）、类黄酮（35.5%）的百分比高于单宁（16.6%）。