Department of Pharmacology, School of Life Dentistry at Tokyo, Nippon Dental University, Tokyo 102-8159, Japan.
Faculty of Life Sciences, Prefectural University of Hiroshima, Hiroshima 727-0023, Japan.
Cells. 2021 Mar 11;10(3):626. doi: 10.3390/cells10030626.
Reactive oxygen species (ROS)-induced oxidative stress in adipose tissue is associated with inflammation and the development of obesity-related metabolic disorders. The aim of this study is to investigate the effects of hydrogen nano-bubble water (HW) on ROS generation, adipogenesis, and interleukin-6 (IL-6) secretion in hydrogen peroxide (HO) or phorbol 12-myristate 13-acetate (PMA)-stimulated OP9 adipocytes, and three-dimensional (3D) subcutaneous adipose equivalents. Nanoparticle tracking analysis showed that fresh HW contains 1.17 × 10/mL of nano-sized hydrogen bubbles. Even after 8 to 13 months of storage, approximately half of the bubbles still remained in the water. CellROX staining showed that HW could diminish HO- or PMA-induced intracellular ROS generation in human keratinocytes HaCaT and OP9 cells. We discovered that PMA could markedly increase lipid accumulation to 180% and IL-6 secretion 2.7-fold in OP9 adipocytes. Similarly, HO (5 µM) also significantly stimulated lipid accumulation in OP9 cells and the 3D adipose equivalents. HW treatment significantly repressed HO- or PMA-induced lipid accumulation and IL-6 secretion in OP9 adipocytes and the 3D adipose equivalents. In conclusion, HW showed a possibility of repressing oxidative stress, inflammatory response, and adipogenesis at cellular/tissue levels. It can be used for preventing the development of metabolic disorders amongst obese people.
活性氧（ROS）诱导的脂肪组织氧化应激与炎症和肥胖相关代谢紊乱的发展有关。本研究旨在探讨纳米氢气泡水（HW）对过氧化氢（HO）或佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）刺激的 OP9 脂肪细胞和成脂分化以及三维（3D）皮下脂肪等同物中 ROS 生成、脂肪生成和白细胞介素-6（IL-6）分泌的影响。纳米颗粒跟踪分析显示，新鲜 HW 含有 1.17×10/mL 的纳米级氢气泡。即使在储存 8 至 13 个月后，水中仍有约一半的气泡存在。CellROX 染色显示，HW 可以减少人角质形成细胞 HaCaT 和 OP9 细胞中 HO 或 PMA 诱导的细胞内 ROS 生成。我们发现 PMA 可以使 OP9 脂肪细胞中的脂质积累显著增加 180%，IL-6 分泌增加 2.7 倍。同样，HO（5μM）也显著刺激 OP9 细胞和 3D 脂肪等同物中的脂质积累。HW 处理显著抑制了 HO 或 PMA 诱导的 OP9 脂肪细胞和 3D 脂肪等同物中的脂质积累和 IL-6 分泌。总之，HW 在细胞/组织水平上显示出抑制氧化应激、炎症反应和脂肪生成的可能性。它可用于预防肥胖人群代谢紊乱的发生。
