Pei Jen-Sheng, Chen Chao-Chun, Chang Wen-Shin, Wang Yun-Chi, Chen Jaw-Chyun, Hsiau Yu-Chen, Hsu Pei-Chen, Hsu Yuan-Nian, Tsai Chia-Wen, Bau Da-Tian
Department of Pediatrics, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan 33004, Taiwan.
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, Taiwan.
Pharmaceuticals (Basel). 2021 Mar 8;14(3):235. doi: 10.3390/ph14030235.
The purpose of our study was to investigate whether genetic variations in lncRNA were associated with susceptibility to childhood leukemia. Two hundred and sixty-six childhood leukemia patients and 266 healthy controls were enrolled in Taiwan, and two single nucleotide polymorphisms (SNPs), rs2839698 and rs217727, in were genotyped and analyzed. There was a significant difference in the genotypic distribution of rs2839698 between patients and healthy controls ( = 0.0277). Compared to the wild-type CC genotype, the heterozygous variant CT and homozygous variant TT genotypes were associated with significantly increased risks of childhood leukemia with an adjusted odd ratio (OR) of 1.46 (95% confidence interval (CI), 1.08-2.14, = 0.0429) and 1.94 (95%CI, 1.15-3.31, = 0.0169), respectively ( = 0.0277). The difference in allelic frequencies between childhood leukemia patients and controls was also significant (T versus C, adjusted OR = 1.53, 95%CI, 1.13-1.79, = 0.0077). There were no significant differences in the genotypic and allelic distributions of rs217727 between cases and controls. Interestingly, the average level of rs2839698 was statistically significantly higher for patients with CT and TT genotypes than from those with the CC genotype ( < 0.0001). Our results indicate that SNP rs2839698, but not rs217727, may serve as a novel susceptibility marker for childhood leukemia.
我们研究的目的是调查长链非编码RNA（lncRNA）的基因变异是否与儿童白血病易感性相关。在台湾招募了266例儿童白血病患者和266名健康对照，对lncRNA中的两个单核苷酸多态性（SNP），即rs2839698和rs217727进行基因分型和分析。患者与健康对照之间rs2839698的基因型分布存在显著差异（P = 0.0277）。与野生型CC基因型相比，杂合变异型CT和纯合变异型TT基因型与儿童白血病风险显著增加相关，校正比值比（OR）分别为1.46（95%置信区间（CI），1.08 - 2.14，P = 0.0429）和1.94（95%CI，1.15 - 3.31，P = 0.0169）（P = 0.0277）。儿童白血病患者与对照之间的等位基因频率差异也很显著（T对C，校正OR = 1.53，95%CI，1.13 - 1.79，P = 0.0077）。病例与对照之间rs217727的基因型和等位基因分布没有显著差异。有趣的是，CT和TT基因型患者的rs2839698平均水平在统计学上显著高于CC基因型患者（P < 0.0001）。我们的结果表明，SNP rs2839698而非rs217727可能作为儿童白血病的一种新的易感性标志物。
