Wójcik-Bojek Urszula, Rywaniak Joanna, Bernat Przemysław, Podsędek Anna, Kajszczak Dominika, Sadowska Beata
Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Molecules. 2021 Mar 21;26(6):1758. doi: 10.3390/molecules26061758.
is still one of the leading causes of both hospital- and community-acquired infections. Due to the very high percentage of drug-resistant strains, the participation of drug-tolerant biofilms in pathological changes, and thus the limited number of effective antibiotics, there is an urgent need to search for alternative methods of prevention or treatment for infections. In the present study, biochemically characterized (HPLC/UPLC-QTOF-MS) acetonic, ethanolic, and water extracts from fruits and bark of L. were tested in vitro as diet additives that potentially prevent staphylococcal infections. The impacts of extracts on sortase A (SrtA) activity (Fluorimetric Assay), staphylococcal protein A (SpA) expression (FITC-labelled specific antibodies), the lipid composition of bacterial cell membranes (LC-MS/MS, GC/MS), and biofilm formation (LIVE/DEAD BacLight) were assessed. The cytotoxicity of extracts to the human fibroblast line HFF-1 was also tested (MTT reduction). extracts strongly inhibited SrtA activity and SpA expression, caused modifications of cell membrane, limited biofilm formation by staphylococci, and were non-cytotoxic. Therefore, they have pro-health potential. Nevertheless, their usefulness as diet supplements that are beneficial for the prevention of staphylococcal infections should be confirmed in animal models in the future.
仍然是医院获得性感染和社区获得性感染的主要原因之一。由于耐药菌株的比例非常高，耐药生物膜参与病理变化，以及有效抗生素数量有限，迫切需要寻找预防或治疗感染的替代方法。在本研究中，对从L.的果实和树皮中提取的经生化表征（HPLC/UPLC-QTOF-MS）的丙酮提取物、乙醇提取物和水提取物进行了体外测试，作为可能预防葡萄球菌感染的饮食添加剂。评估了提取物对分选酶A（SrtA）活性（荧光测定法）、葡萄球菌蛋白A（SpA）表达（异硫氰酸荧光素标记的特异性抗体）、细菌细胞膜的脂质组成（LC-MS/MS、GC/MS）以及生物膜形成（LIVE/DEAD BacLight）的影响。还测试了提取物对人成纤维细胞系HFF-1的细胞毒性（MTT还原法）。提取物强烈抑制SrtA活性和SpA表达，引起细胞膜的改变，限制葡萄球菌生物膜的形成，且无细胞毒性。因此，它们具有促进健康的潜力。然而，它们作为对预防葡萄球菌感染有益的饮食补充剂的有效性，未来应在动物模型中得到证实。
