D'Aquila Patrizia, Ronchetti Domenica, Gallo Cantafio Maria Eugenia, Todoerti Katia, Taiana Elisa, Fabiani Fernanda, Montesanto Alberto, Neri Antonino, Passarino Giuseppe, Viglietto Giuseppe, Bellizzi Dina, Amodio Nicola
Department of Cell Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy.
Department of Oncology and Hemato-Oncology, University of Milan, 20122 Milan, Italy.
J Clin Med. 2021 Mar 21;10(6):1295. doi: 10.3390/jcm10061295.
The mitochondrial quality control network includes several epigenetically-regulated genes involved in mitochondrial dynamics, mitophagy, and mitochondrial biogenesis under physiologic conditions. Dysregulated expression of such genes has been reported in various disease contexts, including cancer. However, their expression pattern and the possible underlying epigenetic modifications remain to be defined within plasma cell (PC) dyscrasias. Herein, we compared the mRNA expression of mitochondrial quality control genes from multiple myeloma, plasma cell leukemia patients and human myeloma cell lines (HMCLs) with healthy plasma cells; moreover, by applying the Sequenom MassARRAY EpiTYPER technology, we performed a pilot investigation of their CpG methylation status in HMCLs. Overall, the results provided indicate dysregulated expression of several mitochondrial network's genes, and alteration of the CpG methylation profile, underscoring novel potential myeloma biomarkers deserving in-depth functional investigation in the future.
线粒体质量控制网络包括几个在生理条件下参与线粒体动力学、线粒体自噬和线粒体生物发生的表观遗传调控基因。在包括癌症在内的各种疾病背景中，已有报道称此类基因表达失调。然而，在浆细胞（PC）发育异常中，它们的表达模式以及可能潜在的表观遗传修饰仍有待确定。在此，我们比较了多发性骨髓瘤、浆细胞白血病患者及人骨髓瘤细胞系（HMCLs）中线粒体质量控制基因与健康浆细胞的mRNA表达；此外，通过应用Sequenom MassARRAY EpiTYPER技术，我们对HMCLs中它们的CpG甲基化状态进行了初步研究。总体而言，所提供的结果表明几个线粒体网络基因表达失调，以及CpG甲基化谱改变，突显了未来值得深入进行功能研究的新型潜在骨髓瘤生物标志物。
