College of Life Science, Sichuan Agricultural University, Ya'an 625000, China.
Int J Mol Sci. 2021 Mar 21;22(6):3197. doi: 10.3390/ijms22063197.
The thick and hard fruit shell of () represents a processing bottleneck. At the same time, soil salinization is one of the main problems faced by modern agricultural production. Bioinformatic analysis indicated that the transcription factor FtNAC16 could regulate the hull cracking of , and the function of this transcription factor was verified by genetic transformation of (). Phenotypic observations of the wild-type (WT), OE-FtNAC16, and -FtNAC16 plant lines confirmed that FtNAC16 negatively regulated pod cracking by downregulating lignin synthesis. Under salt stress, several physiological indicators (POD, GSH, Pro and MDA) were measured, leaves were stained with NBT (Nitroblue Tetrazolium) and DAB (3,3'-diaminobenzidine), and all genes encoding enzymes in the lignin synthesis pathway were analyzed. These experiments confirmed that FtNAC16 increased plant sensitivity by reducing the lignin content or changing the proportions of the lignin monomer. The results of this study may help to elucidate the possible association between changes in lignin monomer synthesis and salt stress and may also contribute to fully understanding the effects of FtNAC16 on plant growth and development, particularly regarding fruit pod cracking and environmental adaptability. In future studies, it may be useful to obtain suitable cracking varieties and salt-tolerant crops through molecular breeding.
()的厚而硬的果实壳代表了一个加工瓶颈。同时，土壤盐碱化是现代农业生产面临的主要问题之一。生物信息学分析表明，转录因子 FtNAC16 可以调节 的荚果开裂，并且通过 ()的遗传转化验证了该转录因子的功能。野生型 (WT)、OE-FtNAC16 和 -FtNAC16 植物系的表型观察证实 FtNAC16 通过下调木质素合成负调控荚果开裂。在盐胁迫下，测量了几种生理指标（POD、GSH、Pro 和 MDA），用 NBT（硝基四氮唑蓝）和 DAB（3,3'-二氨基联苯胺）染色 叶片，并分析了木质素合成途径中所有编码酶的基因。这些实验证实，FtNAC16 通过降低木质素含量或改变木质素单体的比例来增加植物的敏感性。本研究的结果可能有助于阐明木质素单体合成与盐胁迫之间的可能联系，并有助于充分了解 FtNAC16 对植物生长和发育的影响，特别是关于果实荚果开裂和环境适应性。在未来的研究中，通过分子育种获得合适的开裂品种和耐盐作物可能会很有用。
